Detection of cortical laminar architecture using manganese-enhanced MRI
Detection of cortical laminar architecture using manganese-enhanced MRI
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DOI：
10.1016/j.jneumeth.2007.08.020
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发表时间：
2008-01
影响因子：
3
通讯作者：
Afonso C. Silva;J. H. Lee;C. W. Wu;J. Tucciarone;G. Pelled;I. Aoki;A. Koretsky
中科院分区：
文献类型：
--
作者：
Afonso C. Silva;J. H. Lee;C. W. Wu;J. Tucciarone;G. Pelled;I. Aoki;A. Koretsky
将啮齿动物体感皮层的锰增强 MRI (MEMRI) 对比度变化与通过组织组织学和对皮层和丘脑施用解剖示踪剂识别的皮层进行比较。在整个皮质厚度上，MEMRI 信号强度在 I 层较低，在 II 层增加，在 III 层减少，直至 IV 层中部，然后再次增加，在 V 层达到峰值，然后在 VI 层减少。 Reeler 小鼠突变体用于确认 MEMRI 对比度中的皮质交替与层状结构相关。与野生型小鼠不同，卷轴皮质在 MEMRI 信号中没有显示出明显的变化，这与组织学载玻片中不存在皮质层一致。 MEMRI 的纤维束追踪能力用于进一步确认分配并证明层流特异性。将 MnCl2 立体定位注射到丘脑腹后核后 12 至 16 小时，与其他大脑区域相比，在初级体感皮层中检测到信号强度总体增加。最大强度投影图像显示出明显明亮的条纹，位于软脑膜表面下方 600-700μm 处，第四层。数据表明，MEMRI 的系统性和束追踪形式对于研究大脑的层状结构都很有用。
Changes in manganese-enhanced MRI (MEMRI) contrast across the rodent somatosensory cortex were compared to the cortical laminae as identified by tissue histology and administration of an anatomical tracer to cortex and thalamus. Across the cortical thickness, MEMRI signal intensity was low in layer I, increased in layer II, decreased in layer III until mid-layer IV, and increased again, peaking in layer V, before decreasing through layer VI. The reeler mouse mutant was used to confirm that the cortical alternation in MEMRI contrast was related to laminar architecture. Unlike in wild-type mice, the reeler cortex showed no appreciable changes in MEMRI signal, consistent with absence of cortical laminae in histological slides. The tract tracing ability of MEMRI was used to further confirm assignments and demonstrate laminar specificity. Twelve to 16h after stereotaxic injections of MnCl2to the ventroposterior thalamic nuclei, an overall increase in signal intensity was detected in primary somatosensory cortex compared to other brain regions. Maximum intensity projection images revealed a distinctly bright stripe located 600–700μm below the pial surface, in layer IV. The data show that both systemic and tract tracing forms of MEMRI are useful for studying laminar architecture in the brain.