Bicuculline dialysis in the retrotrapezoid nucleus (RTN) region stimulates breathing in the awake rat
Bicuculline dialysis in the retrotrapezoid nucleus (RTN) region stimulates breathing in the awake rat
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DOI：
10.1016/s0034-5687(00)00212-7
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发表时间：
2001-02-01
期刊：
影响因子：
--
通讯作者：
Li, AH
中科院分区：
文献类型：
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作者：
Nattie, E;Shi, J;Li, AH
在清醒大鼠的后斜方核（RTN）区域中的蝇蕈醇透析减少空气呼吸期间的潮气量并降低化学感受（Nattie，Li，2000.应用生理学杂志，89，153-162）。RTN是否与延髓呼吸和加压神经元一样存在内源性GABA能抑制？将荷包牡丹碱微透析（30分钟; 1 mM）进入清醒大鼠的RTN区域，在10至60分钟期间可逆地增加潮气量11-16%（P < 0.01; 6只大鼠）。通气增加，但仅在5、20和25 min时具有显著性（P < 0.05），因为透析期间频率趋于降低。对7% CO2的缓解反应未受影响（6只大鼠）;仅透析溶剂4小时以上无影响（5只大鼠）。II的结论是，在清醒的大鼠有正在进行的内源性调制RTN的影响潮气量的GABA能过程的起源不明。对全身性高碳酸血症的反应缺乏影响表明RTN通过独立于其在化学感受中的作用的过程提供了持续的内源性呼吸驱动。(C)2001爱思唯尔科技有限公司。保留所有权利。
Muscimol dialysis in the retrotrapezoid nucleus (RTN) region of awake rats reduces tidal volume during air breathing and decreases chemoreception (Nattie, Li, 2000. J. Appl. Physiol., 89, 153-162). Is there an endogenous GABAergic inhibition of the RTN as for medullary respiratory and presser neurons? Bicuculline microdialysis (30 min; 1 mM) into the RTN region of awake rats reversibly increased tidal volume by 11-16% over the period from 10 to 60 min (P < 0.01; six rats). Ventilation increased but this was significant (P < 0.05) only at 5, 20, and 25 min as frequency tended to decrease during dialysis. The ventilatory response to 7% CO2 was unaffected (six rats); dialysis of vehicle alone over 4 h had no effect (five rats). II was concluded that in the awake rat there is ongoing endogenous modulation of RTN effects on tidal volume by a GABAergic process of unknown origin. The lack of effect on the response to systemic hypercapnia suggests that the RTN provides an ongoing endogenous drive to respiration by a process that is independent of its role in chemoreception. (C) 2001 Elsevier Science B.V. All rights reserved.