In breast carcinoma tissue, immature dendritic cells reside within the tumor, whereas mature dendritic cells are located in peritumoral areas.
In breast carcinoma tissue, immature dendritic cells reside within the tumor, whereas mature dendritic cells are located in peritumoral areas.
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DOI：
10.1084/jem.190.10.1417
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发表时间：
1999-11-15
期刊：
影响因子：
--
通讯作者：
Banchereau J
中科院分区：
文献类型：
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作者：
Bell D;Chomarat P;Broyles D;Netto G;Harb GM;Lebecque S;Valladeau J;Davoust J;Palucka KA;Banchereau J
我们已经分析了存在的未成熟和成熟的树突状细胞（DC）内腺癌的乳腺癌使用免疫组化。未成熟的DC定义为表达CD 1a-，Langerin-和细胞内主要组织相容性复合物II类丰富的囊泡。通过CD 83和DC-Lamp的表达来确定成熟DC。乳腺癌细胞通过形态学和/或细胞角蛋白表达来定义。我们证明了浸润乳腺癌组织的DC的两种水平的异质性：（a）未成熟的CD 1a + DC，主要是朗格汉斯细胞类型（Langerin+），在32/32个样品中保留在肿瘤床内，（B）成熟的DC，CD 83 +DC-Lamp+，存在于20/32个样品中，局限于肿瘤周围区域。在肿瘤中发现的大量未成熟DC可能最好解释为几乎所有肿瘤细胞均表达高水平的巨噬细胞炎性蛋白3α。说明未成熟/成熟DC区室化模式，体外产生的未成熟DC粘附于肿瘤细胞，而成熟DC选择性粘附于肿瘤周围区域。在某些情况下，T细胞聚集在肿瘤周围区域的成熟DC周围，因此类似于次级淋巴器官的DC-T细胞簇，这是正在进行的免疫反应的特征。
We have analyzed the presence of immature and mature dendritic cells (DCs) within adenocarcinoma of the breast using immunohistochemistry. Immature DCs were defined by expression of CD1a-, Langerin-, and intracellular major histocompatibility complex class II–rich vesicles. Mature DCs were defined by expression of CD83 and DC-Lamp. Breast carcinoma cells were defined by morphology and/or cytokeratin expression. We demonstrate two levels of heterogeneity of DCs infiltrating breast carcinoma tissue: (a) immature CD1a+ DCs, mostly of the Langerhans cell type (Langerin+), were retained within the tumor bed in 32/32 samples and (b) mature DCs, CD83+DC-Lamp+, present in 20/32 samples, are confined to peritumoral areas. The high numbers of immature DCs found in the tumor may be best explained by high levels of macrophage inflammatory protein 3α expression by virtually all tumor cells. Confirming the immature/mature DC compartmentalization pattern, in vitro–generated immature DCs adhere to the tumor cells, whereas mature DCs adhere selectively to peritumoral areas. In some cases, T cells are clustering around the mature DCs in peritumoral areas, thus resembling the DC–T cell clusters of secondary lymphoid organs, which are characteristic of ongoing immune reactions.