Plasma unbound free fatty acid profiles in premature infants before and after intralipid infusion.
Plasma unbound free fatty acid profiles in premature infants before and after intralipid infusion.
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早产儿输注脂肪乳之前和之后的血浆未结合游离脂肪酸谱。
DOI：
10.1080/14767058.2018.1548599
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Kleinfeld,AlanM
中科院分区：
文献类型：
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作者：
Hegyi,Thomas;Weinberger,Barry;Memon,Naureen;Carayannopoulos,Mary;Huber,AndrewH;Kleinfeld,AlanM
背景：未结合的游离脂肪酸（FFAu）是血浆游离脂肪酸（FFA）的生物活性部分。大多数血浆 FFA 与白蛋白结合。只有当 FFA 与白蛋白解离时，它们才会具有生物活性。 目的：测量人类婴儿的第一个 FFAu 谱，并在静脉注射大豆脂质、脂肪乳 (IL) 之前和期间测量这些谱。研究设计：研究对象为 16 名早产儿，来自对 130 名出生体重为 500-2000 g、胎龄为 23-34 周的婴儿进行的家长研究。所选婴儿在出生第一天的血浆样本≥120 µL（每次 FFAu 轮廓测量所需的体积）。婴儿从出生第二天开始接受 IL 输注，剂量为 1 g/kg/天，每天增加 1 g/kg/天，直至 3 g/kg/天。当血浆可用时，在 IL 输注期间测定 FFAu 谱。曲线是九种最丰富的长链 FFAu 的浓度，并使用新型荧光探针测定。结果：在脂肪乳输注之前，未结合的肉豆蔻酸是主要的 FFAu，高达总 FFAu（9 种 FFAu 的总和）的 78%。相比之下，所有婴儿中未结合的亚油酸含量均为 0%。随着 IL 输注量增加至 3 g/kg/天，未结合的亚油酸增加至总 FFAu 的 26%，其中未结合的油酸、肉豆蔻酸和亚麻酸位居第二、第三和第四。平均总 FFAu 浓度也从脂肪乳前的 4nM 增加到 3g/kg/天的 53nM。 IL 输注期间，FFA 谱接近 3 g/kg/天的脂肪乳的脂肪酸组成。结论：这项针对新生儿 FFAu 谱的首次研究表明，在 IL 输注之前，所有 16 名婴儿中未结合的亚油酸为零，肉豆蔻酸水平异常高，高达总 FFAu 谱的 78%。这些结果表明脂质代谢在早期发育中具有重要且以前未被认识的作用。在IL输注之前零未结合的亚油酸可能有助于促进动脉导管的闭合，但在IL输注之后，从亚油酸合成花生四烯可能倾向于促进通畅。可能需要高水平的未结合肉豆蔻酸酯来满足新生儿即时的能量需求。
Background:Unbound free fatty acids (FFAu) are the bioactive fraction of plasma free fatty acids (FFA). Most plasma FFA are bound to albumin. Only when FFA dissociate from albumin, do they become biologically active.Objective:To measure the first FFAu profiles in human infants and to measure these profiles before and during intravenous administration of the soybean lipid, intralipid (IL).Study design:The study population was 16 premature infants, from a parent study of 130 infants with birth weights 500–2000 g and gestational age 23–34 weeks. The infants chosen had plasma samples of ≥120 µL (volume needed for each FFAu profile measurement) in the first day of life. Infants received IL infusions starting in the second day of life at 1 g/kg/day, increasing by 1-g/kg/day daily up to 3 g/kg/day. FFAu profiles were determined during IL infusion when plasma was available. Profiles are the concentrations of the nine most abundant long-chain FFAu and were determined using novel fluorescent probes.Results:Before intralipid infusion unbound myristic acid was the dominant FFAu, as high as 78% of the total FFAu (sum of the 9 FFAu). In contrast, unbound linoleic acid was 0% in all infants. With increasing infusion of IL to 3 g/kg/day, unbound linoleic increased to 26% of the total FFAu, with unbound oleic, myristic, and linolenic acid the second, third and fourth most abundant. The average total FFAu concentration also increased from 4 nM before intralipid to 53 nM at 3 g/kg/day. During IL infusion the FFAu profiles approached the fatty acid composition of intralipid at 3 g/kg/day.Conclusions:This first study of FFAu profiles in neonates revealed that before IL infusion unbound linoleic acid was zero in all 16 infants and levels of myristic acid were exceptionally large, as much as 78% of the total FFAu profile. These results suggest important and previously unrecognized roles of lipid metabolism in early development. Zero unbound linoleic acid before IL infusion may help promote closure of the ductus arteriosus but after IL infusion, synthesis of arachidonic from linoleic acid may tend to promote patency. The high levels of unbound myristate may be needed for immediate neonatal energy needs.