Delimitation of the species of the Debaryomyces hansenii complex by intron sequence analysis
Delimitation of the species of the Debaryomyces hansenii complex by intron sequence analysis
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DOI：
10.1099/ijs.0.004325-0
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发表时间：
2009-05-01
影响因子：
2.8
通讯作者：
Casaregola, Serge
中科院分区：
文献类型：
--
作者：
Jacques, Noemie;Mallet, Sandrine;Casaregola, Serge
使用基于基因谱系的方法来检查分配给汉逊德巴利酵母的菌株之间的物种划分，以比较在四个管家基因（ACT 1，TUB2，RPL 31和RPL 33）中发现的剪接体内含子序列。这揭示了四个不同的菌株群，分别含有D。汉塞尼湾hansenii CBS 767（T），D.汉塞尼湾Fabryi CBS 789（T）、Candida famata var. flareri CBS 1796（T）（D.汉塞尼湾Fabryi CBS 789（T））和Debaryomyces tyrocola CBS 766（T），其种地位不再被接受。这些组之间的序列差异，在某些情况下达到20%以上，明确地将这些组分离为单独的分类群，导致了恢复最初描述的种D的建议。hansenii CBS 767（T）和D. Tyrocola CBS 766（T）.品种D.汉塞尼湾Fabryi进一步细分为两个分类群，Debaryomyces fabryi CBS 789（T）和Candida flareri CBS 1796（T）（以前称为C.法马塔湾flareri和Blastodendrion flareri）。因此，内含子序列的比较暴露了其表型特征与已知物种无法区分的神秘物种，但从遗传学的角度来看，这些物种有显着的分歧。CBS 5571（T）与上述德巴利酵母属物种有亲缘关系，但又明显不同。这项工作中使用的方法也揭示了新划定的物种D中存在种群。hansenii和这些人口之间的遗传交流，这表明一个意想不到的遗传多样性在这一部分的属德巴利酵母。
The delineation of species among strains assigned to Debaryomyces hansenii was examined using a gene genealogies-based approach in order to compare spliceosomal intron sequences found in four housekeeping genes (ACT1, TUB2, RPL31 and RPL33). This revealed four distinct groups of strains containing, respectively, D. hansenii var. hansenii CBS 767(T), D. hansenii var. fabryi CBS 789(T), Candida famata var. flareri CBS 1796(T) (the anamorph of D. hansenii var. fabryi CBS 789(T)) and Debaryomyces tyrocola CBS 766(T), whose species status was no longer accepted. The sequence divergence between these groups, reaching in some cases over 20%, unambiguously isolated the groups as separate taxa, leading to a proposal for the reinstatement of the originally described species D. hansenii CBS 767(T) and D. tyrocola CBS 766(T). The variety D. hansenii var. fabryi was further subdivided into two taxa, Debaryomyces fabryi CBS 789(T) and Candida flareri CBS 1796(T) (previously C. famata var. flareri and Blastodendrion flareri). The comparison of intron sequences therefore exposed cryptic species whose phenotypic traits are not distinguishable from known species, but which have significantly diverged from the genetic point of view. Hence, we describe the new taxon Debaryomyces macquariensis sp. nov. CBS 5571(T) is related to, but clearly distinct from, the Debaryomyces species mentioned above. The approach used in this work has also revealed the existence of populations within the newly delineated species D. hansenii and genetic exchanges between these populations, indicating an unexpected genetic diversity within this part of the genus Debaryomyces.