DOPAMINE-RECEPTORS IN HUMAN-BRAIN - AUTORADIOGRAPHIC DISTRIBUTION OF D2 SITES
DOPAMINE-RECEPTORS IN HUMAN-BRAIN - AUTORADIOGRAPHIC DISTRIBUTION OF D2 SITES
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DOI：
10.1016/0306-4522(89)90179-6
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发表时间：
1989-01-01
期刊：
影响因子：
3.3
通讯作者：
PALACIOS, JM
中科院分区：
文献类型：
--
作者：
CAMPS, M;CORTES, R;PALACIOS, JM
我们已经研究了详细的解剖分布的D2受体在人死后脑组织中使用定量放射自显影技术。使用特异性D2激动剂[3 H]CV 205-502和拮抗剂[3 H]螺哌利多标记D2受体。用两种配体观察到的D2受体分布模式非常相似。密度最高的是尾状核、壳核、延髓核和嗅结节，其次是黑质，D2受体主要集中在延髓部。较低，但仍然显着的密度与苍白球的外侧部分和海马CA 1和CA 3领域。苍白球内侧部、齿状回和杏仁核的D2受体密度低至极低。在嗅球、间脑、脑干、小脑和新皮质的大部分中观察到几乎可忽略不计的结合量。我们的研究结果与以前报道的D2受体在人类和大鼠大脑中的定位。我们还报告了缺乏所谓的螺环癸酮结合位点在人脑中。将D2受体的定位与D1受体的分布进行比较。
We have studied the detailed anatomical distribution of D2 receptors in human post mortem brain tissue using quantitative autoradiographic techniques. D2 receptors were labeled using the specific D2 agonist [3H]CV 205-502 and the antagonist [3H]spiroperidol. The pattern of D2 receptor distribution observed with the two ligands was very similar. The highest densities were found in the nucleus caudatus, putamen, nucleus accumbens and olfactory tubercle followed by the substantia nigra, where D2 receptors were mainy concentrated in the pars compacta. Lower but still significant densities were associated with the lateral part of the globus pallidus and CA1 and CA3 fields of the hippocampus. The medial part of the globus pallidus, the dentate gyrus and the amygdala showed low to very low densities of D2 receptors. Almost negligible amounts of bindings were observed in the olfactory bulb, diencephalon, brainstem, cerebellum and most parts of the neocortex. Our results are comparable with previously reported localizations of D2 receptors in the human and rat brain. We also report the lack of the so-called spirodecanone binding sites in the human brain. The localization of D2 receptors is compared with the distribution of D1 receptors.