The C. elegans gonadal sheath Sh1 cells extend asymmetrically over a differentiating germ cell population in the proliferative zone.
The C. elegans gonadal sheath Sh1 cells extend asymmetrically over a differentiating germ cell population in the proliferative zone.
复制标题
线虫性腺鞘 Sh1 细胞在增殖区的分化生殖细胞群中不对称地延伸。
DOI：
10.7554/elife.75497
复制
发表时间：
2022-09-12
期刊：
影响因子：
7.7
通讯作者：
Gordon, Kacy Lynn
中科院分区：
文献类型：
--
作者：
Li, Xin;Singh, Noor;Miller, Camille;Washington, India;Sosseh, Bintou;Gordon, Kacy Lynn
秀丽隐杆线虫的雌雄同体生殖系被一个由体细胞鞘细胞形成的细管所包围，体细胞鞘细胞支持生殖细胞从干细胞样有丝分裂状态成熟，经过减数分裂、配子发生和排卵。最近，我们发现远端Sh1鞘细胞与有丝分裂的生殖细胞在离开小生境时相关联。2020.在这里，我们报告说，这些鞘相关的生殖细胞首先分化的动物与温度敏感性突变影响生殖细胞的状态，和干细胞样生殖细胞保持远端的Sh1边界。我们分析了几个标记的远端鞘，这是最好的可视化与内源性标记的膜蛋白，过度表达的荧光蛋白未能本地化到远端膜过程，并可能导致性腺形态缺陷。然而，这种具有高度可变表达的试剂可用于确定两个Sh1细胞的相对位置，其中一个细胞通常比另一个细胞延伸得更远。
The Caenorhabditis elegans adult hermaphrodite germline is surrounded by a thin tube formed by somatic sheath cells that support germ cells as they mature from the stem-like mitotic state through meiosis, gametogenesis, and ovulation. Recently, we discovered that the distal Sh1 sheath cells associate with mitotic germ cells as they exit the niche Gordon et al., 2020. Here, we report that these sheath-associated germ cells differentiate first in animals with temperature-sensitive mutations affecting germ cell state, and stem-like germ cells are maintained distal to the Sh1 boundary. We analyze several markers of the distal sheath, which is best visualized with endogenously tagged membrane proteins, as overexpressed fluorescent proteins fail to localize to distal membrane processes and can cause gonad morphology defects. However, such reagents with highly variable expression can be used to determine the relative positions of the two Sh1 cells, one of which often extends further distal than the other.