Expression of MUK/DLK/ZPK, an activator of the JNK pathway, in the nervous systems of the developing mouse embryo
Expression of MUK/DLK/ZPK, an activator of the JNK pathway, in the nervous systems of the developing mouse embryo
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DOI：
10.1016/j.modgep.2004.12.002
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发表时间：
2005-04-01
影响因子：
1.2
通讯作者：
Ohno, S
中科院分区：
文献类型：
--
作者：
Hirai, S;Kawaguchi, A;Ohno, S
C-Jun N-末端激酶（JNK）参与调节神经发育过程中的各种细胞事件，包括分化、凋亡和迁移。MUK/DLK/ZPK是MAP激酶（MAPKKK）酶，其通过MAP激酶激酶（MAPKK）如MKK 7激活JNK。我们发现MUK/DLK/ZPK蛋白在发育中的小鼠胚胎中的表达几乎完全特异于神经组织，包括中枢、外周和自主神经系统。唯一明显的例外是肝脏，其中蛋白质在E11左右暂时表达。在胚胎第10天（E10），神经元产生开始后，在脑和神经嵴组织的神经元中表达变得明显。到E14时，MUK/DLK/ZPK蛋白在各种神经组织中发现，包括脑、脊髓、感觉神经节（例如三叉神经节和背根神经节）以及交感神经和内脏神经。MUK/DLK/ZPK蛋白在神经细胞中的定位几乎始终与β III-微管蛋白（神经元特异性微管蛋白同种型）重叠，并且这两种蛋白质在轴突中比在细胞体和树突中更集中。β III-微管蛋白和MUK/DLK/ZPK的强烈重叠定位表明该蛋白激酶与神经元的微管紧密相关。(c)2005 Elsevier B. V.保留所有权利。
C-Jun N-terminal kinase (JNK) is implicated in regulating the various cellular events during neural development that include differentiation, apoptosis and migration. MUK/DLK/ZPK is a MAP kinase kinase kinase (MAPKKK) enzyme that activates JNK via MAP kinase kinases (MAPKK) such as MKK7. We show here that the expression of MUK/DLK/ZPK protein in the developing mouse embryo is almost totally specific for the neural tissues, including central, peripheral, and autonomic nervous systems. The only obvious exception is the liver, in which the protein is temporally expressed at around E11. The expression becomes obvious in the neurons of the brain and neural crest tissues at embryonic day 10 (E10) after neuron production is initiated. By E14, MUK/DLK/ZPK proteins are found in various neural tissues including the brain, spinal cord, sensory ganglia (such as trigeminal and dorsal root ganglia), and the sympathetic and visceral nerves. The localization of MUK/DLK/ZPK protein in neural cells almost consistently overlapped that of beta III-tubulin, a neuron specific tubulin isoform, and both proteins were more concentrated in axons than in cell bodies and dendrites. The intensely overlapping localization of beta III-tubulin and MUK/DLK/ZPK indicated that this protein kinase is tightly associated with the microtubules of neurons. (c) 2005 Elsevier B.V. All rights reserved.