Identification of Staphylococcus intermedius Group by MALDI-TOF MS
Identification of Staphylococcus intermedius Group by MALDI-TOF MS
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DOI：
10.1016/j.syapm.2010.11.004
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发表时间：
2011-02-01
影响因子：
3.4
通讯作者：
Petrini, Orlando
中科院分区：
文献类型：
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作者：
Decristophoris, Paola;Fasola, Amy;Petrini, Orlando
中间葡萄球菌群包括S.中间S. pseudintermedius和S. Delphini，通常从动物中分离的凝固酶阳性细菌。目前使用分子方法鉴定属于这一组的生物。本研究评估了MALDI-TOF MS用于其鉴定的适用性。69株不同生物学和地理来源的菌株，经hsp 60基因部分测序鉴定为S. intermedius（n = 15）、S. pseudintermedius（n = 32）和S.采用MALDI-TOF MS对22例Delphini标本进行分析，计算其敏感性、特异性和效率。此外，我们还计算了MALDI-TOF MS鉴定结果与部分hsp 60基因测序结果之间的一致性。MALDI-TOF MS对S. [0.95（95% CI：0.68-0.99）]，而中间型链球菌则无显著性差异。假中间型[0.78（95% CI：0.60-0.90）]和S. delphini [0.64（95% CI：0.41-0.83）]，而S. intermedius和S. delphini和S.伪中间体Cohen’s kappa系数表明，MALDI-TOF MS和hsp 60基因测序鉴定S.中间型[0.96（95% CI：0.87-1.04）]，与S. delphini和S.假中间肌[分别为0.70（95% CI：0.52-0.89）和0.76（95% CI：0.616-0.92）]。蛋白质组学鉴定的总体效率范围为0.88（95% CI：0.78-0.95）。pseudintermedius和S. delphini和S.中间体因此，MALDI-TOF MS是一种有价值的和可靠的工具，用于快速和准确地鉴定属于S。intermedius Group. (C)2011年Elsevier GmbH。All rights reserved.
The Staphylococcus intermedius Group includes S. intermedius, S. pseudintermedius and S. delphini, coagulase-positive bacteria commonly isolated from animals. The identification of organisms belonging to this group is presently carried out using molecular methods. This study assessed the suitability of MALDI-TOF MS for their identification. 69 strains of different biological and geographic origins, identified by partial hsp60 gene sequencing as S. intermedius (n = 15), S. pseudintermedius (n = 32) and S. delphini (n = 22), were analyzed by MALDI-TOF MS. The estimated sensitivity, specificity and efficiency were calculated. In addition we computed the agreement between the outcome of MALDI-TOF MS identification and partial hsp60 gene sequencing. The sensitivity of MALDI-TOF MS was higher for S. intermedius [0.95 (95% CI: 0.68-0.99)], than for S. pseudintermedius [0.78 (95% CI: 0.60-0.90)] and S. delphini [0.64 (95% CI: 0.41-0.83)], whereas the specificity was 1 for S. intermedius and S. delphini and 0.97(95% CI: 0.86-0.99) for S. pseudintermedius. The Cohen's kappa coefficient indicated almost perfect agreement between MALDI-TOF MS and hsp60 gene sequencing for the identification of S. intermedius [0.96 (95% CI: 0.87-1.04)], and substantial agreement for S. delphini and S. pseudintermedius [0.70 (95% CI: 0.52-0.89) and 0.76 (95% CI: 0.616-0.92), respectively]. The overall efficiency of the proteomic identification ranged between 0.88 (95% CI: 0.78-0.95) for S. pseudintermedius and S. delphini and 0.99 (95% CI: 0.92-0.99) for S. intermedius. MALDI-TOF MS is thus a valuable and reliable tool for the rapid and accurate identification of bacteria belonging to the S. intermedius Group. (C) 2011 Elsevier GmbH. All rights reserved.