Changes of choroidal structure after treatment for primary intraocular lymphoma: retrospective, observational case series.
Changes of choroidal structure after treatment for primary intraocular lymphoma: retrospective, observational case series.
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DOI：
10.1186/s12886-015-0127-7
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发表时间：
2015-10-19
影响因子：
2
通讯作者：
Sakamoto T
中科院分区：
文献类型：
--
作者：
Egawa M;Mitamura Y;Sano H;Akaiwa K;Niki M;Semba K;Sonoda S;Sakamoto T
我们报告原发性眼内淋巴瘤（PIOL）治疗后，通过增强深度成像光学相干断层扫描（EDI-OCT）图像的二值化确定脉络膜结构的变化。4例PIOL患者的5只眼在玻璃体内注射甲氨蝶呤之前和6个月后进行EDI-OCT检查。另外，对15名年龄和屈光不正控制的正常人的15只眼睛进行EDI-OCT检查，使用公共可访问的软件（ImageJ）对EDI-OCT图像进行二值化。中心凹下脉络膜的检查区域为1，500 μm宽，通过Niblack方法追踪代表管腔区域的暗区。Wilcoxon符号秩检验用于确定中心凹下脉络膜厚度、间质面积和管腔面积变化的显著性。采用Mann-Whitney U检验比较术前植入PIOL眼和正常对照眼的各项参数。治疗后中心凹下脉络膜厚度明显降低（P = 0.0431）。在二值化图像中，治疗后间质面积显著减少（P = 0.0431），而管腔面积无显著变化（P = 0.8927）。PIOL延迟发作后，1只眼观察到间质面积增加，脉络膜增厚，管腔面积不变。与正常对照眼相比，PIOL预处理眼的间质面积和脉络膜厚度显著增加（分别为P = 0.0207，P = 0.0495），而腔面积无显著差异（P = 0.2752）。PIOL治疗后，EDI-OCT图像显示脉络膜变薄，EDI-OCT图像的二值化显示与管腔区域相比，间质区域显著减少。二值化的EDI-OCT图像可以提供关于患有PIOL的眼睛中的脉络膜结构的有用信息，并且将这些图像与眼内白细胞介素水平或眼底自发荧光图像相结合应该为确定PIOL活性提供有价值的信息。
We report changes of choroidal structure determined by binarization of enhanced depth imaging optical coherence tomographic (EDI-OCT) images after treatment for primary intraocular lymphoma (PIOL). Five eyes of four patients with PIOL were examined by EDI-OCT before and 6 months after intravitreal methotrexate injections. In addition, 15 eyes of 15 normal individuals controlled by age and refractive error were examined by EDI-OCT. Binarization of the EDI-OCT images was performed using publicly accessible software (ImageJ). The examined area of the subfoveal choroid was 1,500 μm wide, and the dark areas that represented the luminal areas were traced by the Niblack method. Wilcoxon signed rank test was used to determine the significance of changes in the subfoveal choroidal thickness, interstitial area, and luminal area. Mann–Whitney U test was used to compare the parameters in the eyes with pretreatment PIOL and normal control eyes. The subfoveal choroidal thickness was significantly decreased after treatment (P = 0.0431). In the binarized images, the interstitial area was significantly decreased after treatment (P = 0.0431), while the luminal area was not significantly changed (P = 0.8927). After delayed onset of PIOL, increased interstitial area, thickened choroid and unchanged luminal area were observed in one eye. The interstitial area and choroidal thickness were significantly increased in the eyes with pretreatment PIOL compared with the normal control eyes (P = 0.0207, P = 0.0495, respectively), while the luminal area was not significantly different (P = 0.2752). After treatment for PIOL, the EDI-OCT images showed a thinner choroid, and binarization of the EDI-OCT images showed significantly decreased interstitial areas compared with the luminal areas. The binarized EDI-OCT images can provide useful information on choroidal structure in eyes with PIOL, and combining these images with intraocular interleukin levels or fundus autofluorescence images should provide valuable information for determining the PIOL activity.