The relationship of tryptase- and chymase-positive mast cells to angiogenesis in stage I non-small cell lung cancer
The relationship of tryptase- and chymase-positive mast cells to angiogenesis in stage I non-small cell lung cancer
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DOI：
10.1016/j.ejcts.2005.06.020
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发表时间：
2005-10-01
影响因子：
3.4
通讯作者：
Miyazaki, M
中科院分区：
文献类型：
--
作者：
Ibaraki, T;Muramatsu, M;Miyazaki, M
目的：人肥大细胞分为类胰蛋白酶阳性肥大细胞（MCT）和类胰蛋白酶和糜蛋白酶阳性肥大细胞（MCTC）。尽管已有研究表明MCT与血管生成有关，但关于MCTC参与肿瘤血管生成的研究却知之甚少。为了阐明MCTC与肺癌血管生成之间的关系，我们评估了正常、边缘和中央型肺癌区域的MCTC、MCT和微血管计数。方法：对32例腺癌和13例鳞癌的肿瘤切片进行了糜酶免疫染色以评估MCTC，类胰蛋白酶免疫染色以评估MCT，CD 34免疫染色以评估微血管计数。边缘区MCTC和MCT计数均显著高于中心区，中心区MCTC和MCT计数显著高于正常区。边缘区微血管计数高于中央区。边缘区MCTC/MCT比值明显高于正常区，而中央区无明显差异。在边缘区，不仅MCT和微血管计数之间存在显著相关性，而且MCTC和微血管计数之间也存在显著相关性。在中央区，MCTC与微血管计数呈显著相关，而MCT与微血管计数无显著相关性。结论：MCTC可能参与了肺癌血管生成的发生。(c)2005年由Elsevier B. V.出版
Objective: There are two types of human mast cells, tryptase-positive mast cells (MCT) and tryptase- and chymase-positive mast cells (MCTC). Although MCT have been reported to be related to the generation of angiogenesis, little is known about the involvement of MCTC in tumor angiogenesis. In this study, to clarify the relationship between MCTC and lung cancer angiogenesis, we evaluated MCTC, MCT, and microvessel counts in normal, border, and central lung cancer regions.Methods: Tumor sections from 32 cases of adenocarcinoma and 13 cases of squamous cell carcinoma were immunostained for chymase to evaluate MCTC, tryptase to evaluate MCT, and CD34 to evaluate microvessel counts.Results: Both MCTC and MCT counts in the border lung cancer region were significantly higher than in the central region, and the MCTC and MCT counts in the central region were significantly higher than those in the normal regions. The microvessel counts in the border region were higher than those in the central region. The ratio of MCTC to MCT in the border region, but not in the central region, was significantly higher than that in the normal region. In the border region, significant correlations not only between MCT and microvessel count, but also between MCTC and microvessel count were, observed. In the central region, a significant correlation between MCTC and the microvessel count was observed, but there was no significant correlation between MCT and the microvessel count.Conclusions: These findings suggest that MCTC may be involved in the pathogenesis of angiogenesis in lung cancer. (c) 2005 Published by Elsevier B.V.