A fluorophore-based bio-barcode amplification assay for proteins
A fluorophore-based bio-barcode amplification assay for proteins
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DOI：
10.1002/smll.200500260
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发表时间：
2006-01-01
期刊：
影响因子：
13.3
通讯作者：
Mirkin, CA
中科院分区：
文献类型：
--
作者：
Oh, BK;Nam, JM;Mirkin, CA
生物标记物的高灵敏度分析对于许多应用至关重要，包括医学诊断、药物开发、法医学、基因组学和蛋白质组学研究。[1-18]在DNA和蛋白质检测领域取得了重大进展，一些新技术即将出现。一些最有前途的是基于新的探针材料，特别是金纳米粒子、纳米线和半导体量子点结构。[1,2,19 - 37]尽管其中许多技术可能会引发基于比色法、电学、电化学或基于拉曼的信号转导方案的新检测形式，但目前诊断界使用的主要技术依赖于分子荧光探针和荧光作为传感模式
High-sensitivity assays for biomarkers are of paramount importance for many applications, including medical diagnostics, pharmaceutical development, forensics, and genomics and proteomics research.[1–18] Significant advances have been made in the area of DNA and protein detection, and several new technologies loom on the horizon. Some of the most promising are based upon novel probe materials, in particular gold nanoparticles, nanowires, and semiconductor quantum dot structures.[1, 2, 19–37]Although many of these technologies may initiate the move to novel forms of detection based upon colorimetric, electrical, electrochemical, or Raman-based signal transduction schemes, the dominant technology currently used by the diagnostics community relies on molecular fluorophore probes and fluorescence as a sensing modal-