Biofabrication and Characterization of Collagens with Different Hierarchical Architectures
Biofabrication and Characterization of Collagens with Different Hierarchical Architectures
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具有不同层次结构的胶原蛋白的生物制造和表征
DOI：
10.1021/acsbiomaterials.9b01252
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发表时间：
2020
影响因子：
--
通讯作者：
Yue Ouyang
中科院分区：
文献类型：
--
作者：
Liu Xinhua;Zheng Manhui;Wang Xuechuan;Luo Xiaomin;Hou Mengdi;Yue Ouyang
本文基于天然胶原的生物合成途径，系统地对胶原分子（Col）、胶原微纤维（F-col）和胶原纤维束（Ag-col）这三类具有不同层次结构的胶原进行了生物制造。综合评价了它们的宏观性质，即理化性质和生物学性质以及分级结构。结果表明，Col具有刚性棒状纤维状三螺旋结构，而F-col和Ag-col具有典型的D周期性横纹结构，其长度分别为54和60 nm。我们进一步发现，具有更高层次结构的胶原蛋白具有更上级的热稳定性，机械性能和生物降解性。在所有三种胶原中，Ag-col是最好的：它具有最高的生物活性和止血性能，并且可以更好地促进细胞粘附、生长和增殖，更好地改善生长因子的分泌。总的来说，我们有理由相信，具有更高层次结构的胶原蛋白可以作为胶原材料的更好的替代来源，用于生物工业中的进一步应用。
Herein, three categories of collagens with different hierarchical architectures, including collagen molecules (Col), collagen microfibrils (F-col), and collagen fiber bundles (Ag-col), were systematically biofabricated based on the biosynthesis pathway of natural collagen. Their macroscopic properties, that is, physicochemical and biological properties, and hierarchical structures were evaluated synthetically. The results showed that Col had a rigid rod-like fibrous triple helix structure, whereas F-col and Ag-col had the typical D-periodic cross-striated patterns with lengths of about 54 and 60 nm in the longitudinal direction, respectively. We further found that collagens with higher hierarchical structures had more superior thermal stability, mechanical properties, and biodegradability. Among all three collagens, Ag-col was the best: it had the highest bioactivity and hemostatic properties and could better promote cell adhesion, growth, and proliferation and better improve the secretion of growth factors. Overall, we have a reason to believe that collagens with a higher hierarchical structure can serve as a better alternative source of collagenous materials for further applications in biological industries.