Characterization of a necrotizing enterocolitis model in newborn mice.
Characterization of a necrotizing enterocolitis model in newborn mice.
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发表时间：
2010-09
影响因子：
0.1
通讯作者：
Runlan Tian;S. Liu;Cara E. Williams;Thomas D. Soltau;R. Dimmitt;Xiaotian Zheng;Isabelle G. De Plaen
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作者：
Runlan Tian;S. Liu;Cara E. Williams;Thomas D. Soltau;R. Dimmitt;Xiaotian Zheng;Isabelle G. De Plaen
背景坏死性小肠结肠炎（NEC）是早产儿的一个主要健康问题，其发病机制仍然知之甚少。目前的小鼠NEC模型尚未得到很好的表征。在这项研究中，我们开发了一种简单的NEC小鼠模型，并确定了几种调节人NEC的因子的作用（即，母乳、出生体重、剖宫产和细菌）对肠道损伤的影响。在第一次实验中，自然出生的幼仔和喂养2级的母鼠分别为70%和37%，而对照组（无细菌）为6%（p<0.05）。在喂食12至24小时的幼仔中，NEC发生率比喂食少于12小时的幼仔低44（±12）%（p<0.05）。我们没有发现自然出生的幼崽之间的NEC发病率喂养不到12小时，剖宫产出生的。重度NEC的发生率在低出生体重的幼仔中较高。结论：我们已经简化并表征了与人类NEC具有几个危险因素的新生小鼠NEC模型。现在，转基因小鼠是可用的，这个模型将是有用的，以研究特定的蛋白质在NEC的发展在体内发挥的作用。
BACKGROUND Necrotizingenterocolitis (NEC) is a major health concern for premature infants and its patho-genesis remains poorly understood. The current mouse NEC model has not well been characterized. OBJECTIVES In this study, we develop a simple mouse model of NEC and determine the role of several factors modulating human NEC (i.e., breast milk, birth weight, cesarean section and bacteria) on intestinal injury. METHODS In a first experiment, pups born naturally and dam fed for grade 2) was 70% and 37% respectively vs 6% in the controls (no bacteria)(p<0.05). In pups dam fed for 12 to 24 hours, NEC incidence was 44(±12)% lower vs those dam fed less than 12 hours (p<0.05). We did not find any difference in the NEC incidence between naturally-born pups dam fed for less than 12 hours and these born by cesarean section. The incidence of severe NEC was higher in pups with low birth weight. CONCLUSIONS we have simplified and characterized a neonatal mouse NEC model that shares several risk factors with human NEC. Now that transgenic mice are available, this model will be useful to study the role played by specific proteins in vivo in NEC development.