Taurine prevents myocardial ischemia/reperfusion-induced oxidative stress and apoptosis in prolonged hypothermic rat heart preservation
Taurine prevents myocardial ischemia/reperfusion-induced oxidative stress and apoptosis in prolonged hypothermic rat heart preservation
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DOI：
10.1007/s00380-005-0841-9
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发表时间：
2005-11-01
影响因子：
1.5
通讯作者：
Komeda, M
中科院分区：
文献类型：
--
作者：
Oriyanhan, W;Yamazaki, K;Komeda, M
牛磺酸（2-氨基乙磺酸）是一种有效的抗氧化剂，可抑制缺血再灌注损伤中的细胞凋亡。本研究旨在探讨在St.托马斯心脏停搏液中加入牛磺酸是否能增强其在大鼠心脏长时间低温保存中的心肌保护作用。将从雄性Sprague-Dawley大鼠分离的心脏固定在Langendorff装置上以估计基线心功能，然后停搏并在4 ℃下储存在含有牛磺酸（10 mM;牛磺酸组，n = 8）或不含牛磺酸（对照组，n = 8）的St.托马斯心脏停搏液中6小时。储存后，心脏进行再灌注，并测量心率（HR）、冠状动脉流量（CF）、左心室发展压（LVP）和正最大左心室发展压（max LV dp/dt）。免疫组化法检测左室组织DNA氧化应激和细胞凋亡。与对照组相比，（P <0.001），最大左心室dp/dt（P <0.001），冠状动脉血流（P <0.001），而谷草转氨酶（P <0.01），乳酸脱氢酶（P <0.05），磷酸肌酸激酶（P <0.01），8-羟基-2'-脱氧鸟苷指数牛磺酸组caspase-3 mRNA表达（P <0.05）和TUNEL阳性细胞百分率（P <0.01）均降低。在St.托马斯心脏停搏液中加入牛磺酸可通过抑制DNA氧化应激和细胞凋亡促进大鼠心脏长期低温保存后的心功能恢复。
Taurine (2-aminoethanesulfonic acid) is a potent antioxidant and inhibits cell apoptosis in ischemic reperfusion injury. In this study we evaluated whether addition of taurine to St. Thomas' cardioplegic solution enhances its myocardial protective effects in prolonged hypothermic heart preservation in rats. Hearts isolated from male Sprague-Dawley rats were mounted on a Langendorff apparatus to estimate baseline cardiac function, then arrested and stored in St. Thomas' cardioplegic solution, with taurine (10 mM; taurine group, n = 8) or without taurine (control group, n = 8), for 6h at 4 C. After storage, the hearts were reperfused and heart rate (HR), coronary flow (CF), left ventricular developed pressure (LVP), and positive maximum left ventricular developing pressure (max LV dp/dt) were measured. The LV tissue was examined immunohistochemically for determining DNA oxidative stress and cell apoptosis. Compared with control groups, recovery of LVP (P < 0.001), max LV dp/dt (P < 0.001), and coronary flow (P < 0.001) were significantly enhanced, whereas glutamic oxaloacetic transaminase (P < 0.01), lactate dehydrogenase (P < 0.05), creatine phosphate kinase (P < 0.01), 8-hydroxy-2'-deoxyguanosine index (P < 0.01), caspase-3 mRNA expression (P < 0.05), and percentage of TUNEL-positive cardiomyocytes (P 0.05) were reduced in the taurine group. Addition of taurine to St. Thomas' cardioplegic solution improved cardiac function recovery for prolonged hypothermic rat heart preservation by suppressing DNA oxidative stress and cell apoptosis.