Characterization of a method for measuring primary hyperalgesia of deep somatic tissue
Characterization of a method for measuring primary hyperalgesia of deep somatic tissue
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DOI：
10.1016/j.jpain.2004.10.002
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发表时间：
2005-01-01
期刊：
影响因子：
4
通讯作者：
Sluka, KA
中科院分区：
文献类型：
--
作者：
Skyba, DA;Radhakrishnan, R;Sluka, KA
在实验动物中测量深部躯体组织(即肌肉和关节)的原发性痛觉过敏是困难的，但在临床上具有重要意义。在这项研究中，我们改进了一种新开发的测量大鼠肌肉原发性痛敏的方法，并将其与膝关节的原发性痛敏进行了比较。用连接在钳子上的应变片测量腓肠肌或膝关节的压缩收缩阈值。用钳子压迫肌肉或关节导致后肢收缩，分别在致炎前和致炎后4h分别测定腓肠肌注射3%角叉菜胶或膝关节注射3%高岭土-角叉菜胶的阈值。肌肉或膝关节炎症诱导4小时后，与炎症前的阈值相比，压缩阈值显著降低。令人惊讶的是，在肌肉发炎的动物中，对侧后肢的压缩阈值也显著降低。全身注射吗啡(5 mg/kg，腹腔注射)或利多卡因(2%)注射到炎症组织中，可逆转由深层组织炎症引起的压缩阈值降低。然而，局部麻醉剂应用于肌肉或膝关节上的皮肤并不影响降低的阈值。因此，我们报道了一种一致而简便的方法来测量大鼠深部组织中的原发性痛敏。测量到的痛觉过敏起源于炎症组织，皮肤没有可测量的贡献。观点：目前的方法直接从损伤的深部躯体组织测量原发性痛敏。因此，它与因损伤部位的机械压力而加重的疼痛临床状况有关。因此，它可能成为肌肉骨骼疼痛综合征的基础机制研究和药物发现的模型。(C)2005年，由美国疼痛协会提供。
Measuring primary hyperalgesia from deep somatic tissue (ie, muscle and joint) is difficult in laboratory animals but clinically important. In this study, we modified a newly developed method to measure primary hyperalgesia of muscle in rats and compared this with primary hyperalgesia from the knee. Compression withdrawal thresholds of the gastrocnemius muscle or the knee joint were measured with a device consisting of strain gauges attached to forceps. Compression of the muscle or joint with the forceps results in hind limb withdrawal, and thresholds were measured before and 4 hours after induction of inflammation by 3% carrageenan injected into the gastrocnemius muscle or 3% kaolin-carrageenan injected into the knee joint. Compression thresholds were significantly decreased 4 hours after induction of inflammation in the muscle or knee joint compared with thresholds before inflammation. Surprisingly, in animals with muscle inflammation, compression thresholds were also significantly decreased on the contralateral hind limb. Systemic morphine (5 mg/kg, intraperitoneal) or lidocaine (2%) injected into the inflamed tissue reversed the decreased compression threshold induced by deep tissue inflammation. However, local anesthetic applied to the skin overlying the muscle or knee joint did not affect the decreased threshold. Thus, we report a consistent and convenient method to measure primary hyperalgesia in deep tissues of rats. The measured hyperalgesia originates in the inflamed tissues and has no measurable contribution from skin.Perspective: The current method measures primary hyperalgesia directly from injured deep somatic tissues. Thus it is relevant to painful clinical conditions that are aggravated by mechanical pressure at the site of injury. As such, it might serve as a model for basic mechanistic studies as well as drug discovery for musculoskeletal pain syndromes. (C) 2005 by the American Pain Society.