PHYSICAL FACTORS ARE INVOLVED IN THE DESTRUCTION OF EMBRYOS AND OOCYTES DURING FREEZING AND THAWING PROCEDURES
PHYSICAL FACTORS ARE INVOLVED IN THE DESTRUCTION OF EMBRYOS AND OOCYTES DURING FREEZING AND THAWING PROCEDURES
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DOI：
10.1093/oxfordjournals.humrep.a136785
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发表时间：
1988-08-01
影响因子：
6.1
通讯作者：
ASHORN, R
中科院分区：
文献类型：
--
作者：
ASHWOODSMITH, MJ;MORRIS, GW;ASHORN, R
在冷冻显微镜下对小鼠、人卵母细胞和小鼠胚胎冷冻和解冻过程的观察表明，物理因素和物理化学因素在解冻后致死性损伤的发生中可能起作用。与接近的冰锋的接触点可能使该区域更容易出现未来的细胞质气泡。冰锋扭曲了卵母细胞，这种扭曲在其随后的热历史过程中仍然存在，与干燥扭曲无关。冰启动了细胞内和细胞外气泡的形成，这些气泡在解冻时是明显的；随着解冻过程的进行，可以看到它们的体积增大。这些气泡的生长会导致扩张的囊泡，这可能会完全摧毁胚胎。讨论了这些物理因素对卵母细胞、胚胎、组织和器官冷冻成功的影响。
Observations made during the freezing and thawing of mouse and human oocytes and mouse embryos with the cryomicroscope suggest that physical factors as well as physicochemical factors may play a role in the development of lethal damage upon thawing. The point of contact with the approaching ice front may predispose that area to the appearance of future cytoplasmic blebbing. The ice front distorts the oocyte and this distortion remains during its subsequent thermal history and is unrelated to desiccation distortion. Ice initiates the formation of both intra- and extracellular gas bubbles which are apparent upon thawing; with the progression of the thawing process they can be seen to grow in volume. Growth of these bubbles can give rise to expanding vesicles which can totally destroy an embryo. The consequences of these physical factors for the successful cryopreservation of oocytes, embryos, tissues and organs are discussed.