Nuclear remodeling and nuclear reprogramming for making transgenic pigs by nuclear transfer.
Nuclear remodeling and nuclear reprogramming for making transgenic pigs by nuclear transfer.
复制标题
通过核移植制造转基因猪的核重塑和核重编程。
DOI：
10.1007/978-0-387-37754-4_1
复制
发表时间：
2007
影响因子：
--
通讯作者：
Prather,RandallS
中科院分区：
文献类型：
--
作者：
Prather,RandallS
更好地了解细胞核转移到卵母细胞的细胞质时发生的细胞和分子事件将允许开发用于执行核转移和克隆的改进程序。在某些情况下，似乎基因被重新编程，而在其他情况下，似乎对基因表达几乎没有影响。不仅基因表达模式需要重新编程，细胞核内的其他结构也需要重塑。虽然核移植工作和转基因和敲除动物可以创造，它仍然是一个效率低下的过程。然而，即使目前效率较低，这种技术已被证明对生产可用于人类组织或器官移植的动物非常有价值。
A better understanding of the cellular and molecular events that occur when a nucleus is transferred to the cytoplasm of an oocyte will permit the development of improved procedures for performing nuclear transfer and cloning. In some cases it appears that the gene(s) are reprogrammed, while in other cases there appears to be little effect on gene expression. Not only does the pattern of gene expression need to be reprogrammed, but other structures within the nucleus also need to be remodeled. While nuclear transfer works and transgenic and knockout animals can be created, it still is an inefficient process. However, even with the current low efficiencies this technique has proved very valuable for the production of animals that might be useful for tissue or organ transplantation to humans.