Mucosal immunoglobulin response in Epinephelus coioides after Cryptocaryon irritans infection
Mucosal immunoglobulin response in Epinephelus coioides after Cryptocaryon irritans infection
复制标题
刺激白点虫感染后斜带石斑鱼的粘膜免疫球蛋白反应
DOI：
10.1016/j.fsi.2022.08.011
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发表时间：
2022-08-19
影响因子：
4.7
通讯作者：
Li, Yanwei
中科院分区：
文献类型：
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作者：
Han, Qing;Mo, Zequan;Li, Yanwei
硬骨鱼的粘膜免疫系统主要由皮肤、鳃和肠道组成，它们在抵抗入侵生物的局部免疫反应中起着至关重要的作用。免疫球蛋白是获得性免疫中执行关键生物学功能的必需分子。本研究借鉴前人的经验，建立了刺激隐核虫感染斜带石斑鱼的粘膜免疫模型。免疫组血清和粘液中总IgM和IgT升高，而仅检测到病原特异性IgM。更重要的是，在头肾、鳃和皮肤上清中检测到病原体特异性IgM，从而表明系统免疫和粘膜免疫系统分泌免疫球蛋白。此外，在检测皮肤上清液中的病原体特异性IgM的基础上，观察到皮肤中的早期应答。本研究初步揭示了石斑鱼鳃和皮肤中IgM和IgT对病原菌的黏膜免疫应答特征，为石斑鱼疫苗的研究提供了理论依据。刺激物。
The teleost mucosal immune system consists mainly of the skin, gills and gut, which play crucial roles in local immune responses against invading organisms. Immunoglobulins are essential molecules in adaptive immunity that perform crucial biological functions. In our study, a mucosal immunity model was constructed in Epinephelus coioides groupers after Cryptocaryon irritans infection, according to previous experience. Total IgM and IgT in the groupers increased in the serum and mucus in the immune group, whereas only pathogen-specific IgM were detected existence. More critically, pathogen-specific IgM was detected in the head kidney, gill and skin su-pernatants, thus suggesting that the systematic immune and mucosal immune system secreted immunoglobulins. Furthermore, an early response in the skin was observed, on the basis of the detection of pathogen-specific IgM in the skin supernatant. In conclusion, this research characterized the grouper IgM and IgT in mucosal immune responses to pathogens in the gills and skin, thus providing a theoretical basis for future studies on vaccines against C. irritans.