Rhamnan sulphate from Monostroma nitidum attenuates hepatic steatosis by suppressing lipogenesis in a diet-induced obesity zebrafish model
Rhamnan sulphate from Monostroma nitidum attenuates hepatic steatosis by suppressing lipogenesis in a diet-induced obesity zebrafish model
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DOI：
10.1016/j.jff.2015.05.041
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发表时间：
2015-08
影响因子：
5.6
通讯作者：
Liqing Zang;Yasuhito Shimada;Toshio Tanaka;Norihiro Nishimura
中科院分区：
文献类型：
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作者：
Liqing Zang;Yasuhito Shimada;Toshio Tanaka;Norihiro Nishimura
硫酸鼠李聚糖（Rhamnan sulfate，RS）是从海洋生物膜（Monostroma nitidum）中分离得到的一种硫酸多糖。在这里，RS对代谢紊乱的影响，使用饮食诱导的肥胖症（DIO）的斑马鱼进行了研究。口服RS（250 µg/g BW/天）可减轻DIO的体重增加、血脂异常（血浆三酰甘油和低密度脂蛋白胆固醇）和肝脂肪变性。在肝组织中，RS降低CCAAT/增强子结合蛋白α（cebpa）和乙酰辅酶A羧化酶1（acacB）的基因表达水平，并增加过氧化物酶体增殖物激活受体α B（ppara B）的表达。而pparab下游基因acadmandacox 1的表达水平并没有增加，提示RS的降脂作用可能主要是通过抑制脂肪生成而实现的。据我们所知，这是第一个研究表明RS具有强大的体内降脂作用。RS作为一种功能性食品的摄入可能适用于预防肥胖和降低肥胖相关疾病的患病率。
Rhamnan sulphate (RS) is a sulphated polysaccharide fromMonostroma nitidum. Here, the effects of RS on metabolic disorders using zebrafish with diet-induced obesity (DIO) were investigated. Oral administration of RS (250 µg/g BW/day) attenuated body weight gain, dyslipidaemia (plasma triacylglycerol and low-density lipoprotein cholesterol) and hepatic steatosis in DIO. In liver tissues, RS reduced gene expression levels of CCAAT/enhancer-binding protein alpha (cebpa) and acetyl-CoA carboxylase 1 (acacb), and increased peroxisome proliferator-activated receptor alpha b (pparab) expression. However, the expression levels of downstream genes ofpparab(acadmandacox1) were not increased, suggesting that the lipid-lowering effect of RS might be caused mainly by inhibition of lipogenesis. To the best of our knowledge, this is the first study to demonstrate that RS has a powerful lipid-lowering effectin vivo. Intake of RS as a functional food may be suitable for obesity prevention and for reducing the prevalence of obesity-related diseases.