Micro-irradiation tools to visualize base excision repair and single-strand break repair
Micro-irradiation tools to visualize base excision repair and single-strand break repair
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DOI：
10.1016/j.dnarep.2015.05.001
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发表时间：
2015-07-01
期刊：
影响因子：
3.8
通讯作者：
Wilson, Samuel H.
中科院分区：
文献类型：
--
作者：
Gassman, Natalie R.;Wilson, Samuel H.
显微镜和微辐射成像技术大大提高了我们对DNA损伤耐受性和DNA修复蛋白在损伤部位的组装的认识。虽然这些工具已被广泛应用于核苷酸切除修复和双链断裂修复的研究，但它们在修复氧化诱导的碱基损伤和单链断裂方面的应用才刚刚开始产生新的见解。本次审查将集中在检查微辐射技术报告创建基地病变和单链断裂，这些病变被认为是主要解决的蛋白质参与的碱基切除修复（BER）途径。通过检查产生这些DNA损伤的条件和审查信息的组装和解离的修复复合物在诱导的损伤部位，我们希望促进BER的进一步调查，并刺激进一步发展和增强这些技术的BER的研究。由爱思唯尔公司出版
Microscopy and micro-irradiation imaging techniques have significantly advanced our knowledge of DNA damage tolerance and the assembly of DNA repair proteins at the sites of damage. While these tools have been extensively applied to the study of nucleotide excision repair and double-strand break repair, their application to the repair of oxidatively-induced base lesions and single-strand breaks is just beginning to yield new insights. This review will focus on examining micro-irradiation techniques reported to create base lesions and single-strand breaks; these lesions are considered to be primarily addressed by proteins involved in the base excision repair (BER) pathway. By examining conditions for generating these DNA lesions and reviewing information on the assembly and dissociation of repair complexes at the induced lesion sites, we hope to promote further investigations into BER and to stimulate further development and enhancement of these techniques for the study of BER. Published by Elsevier B.V.