Polymer-enhanced adenoviral transduction of CAR-negative bladder cancer cells.
Polymer-enhanced adenoviral transduction of CAR-negative bladder cancer cells.
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DOI：
10.1021/mp9000958
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发表时间：
2009-09
影响因子：
4.9
通讯作者：
Voelkel-Johnson C
中科院分区：
文献类型：
--
作者：
Kasman LM;Barua S;Lu P;Rege K;Voelkel-Johnson C
腺病毒基因治疗癌症的应用受到病毒免疫清除和较差的转导效率的限制，因为用于病毒进入（CAR）的蛋白质在粘附中起生理作用，并且在癌细胞中经常减少。阳离子聚合物已被用于增强腺病毒基因传递，但需要低毒性的新型聚合物来实现这一方法。我们最近发现的聚合物具有质粒DNA转染效率高和毒性低的特点。在这项研究中，我们评估了新型阳离子聚合物egde -3,3 '增加car阴性膀胱癌细胞系TCCSUP腺病毒转导的潜力。转染50-60%的细胞所需的腺病毒数量减少了100倍。GFP与egde -3,3 '聚合物预孵育。聚乙烯亚胺（pEI）是一种带正电荷的聚合物，目前被用作增强腺病毒转导的标准，它也增加了传染性，但egde -3,3 '的转基因表达始终较高。此外，egde -3,3 '补充了表达诱导凋亡的转基因Ad的腺病毒的转导。GFP-TRAIL显著增加细胞死亡数量。因此，我们的研究结果表明，新的生物相容性聚合物可能有助于改善腺病毒基因治疗的递送。
The application of adenoviral gene therapy for cancer is limited by immune clearance of the virus as well as poor transduction efficiency, since the protein used for viral entry (CAR) serves physiological functions in adhesion and is frequently decreased among cancer cells. Cationic polymers have been used to enhance adenoviral gene delivery but novel polymers with low toxicity are needed to realize this approach. We recently identified polymers that were characterized by high transfection efficiency of plasmid DNA and a low toxicity profile. In this study we evaluated the novel cationic polymer EGDE-3,3′ for its potential to increase adenoviral transduction of the CAR-negative bladder cancer cell line TCCSUP. The amount of adenovirus required to transduce 50-60% of the cells was reduced 100-fold when Ad.GFP was pre-incubated with the EGDE-3,3′ polymer. Polyethyleneimine (pEI), a positively charged polymer currently used as a standard for enhancing adenoviral transduction, also increased infectivity, but transgene expression was consistently higher with EGDE-3,3′. In addition, EGDE-3,3′-supplemented transduction of an adenovirus expressing an apoptosis inducing transgene, Ad.GFP-TRAIL, significantly enhanced the amount of cell death. Thus, our results indicate that novel biocompatible polymers may be useful in improving the delivery of adenoviral gene therapy.
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影响因子：
10.8
作者：
Jung, Yukyung;Park, Hyo-Jin;Haam, Seungjoo
通讯作者：
Haam, Seungjoo
DOI：
10.1590/s0104-14282008000100015
发表时间：
2008-03-01
期刊：
Polímeros
影响因子：
--
作者：
Loos, Marcio R.;Coelho, Luiz Antonio F.;Amico, Sandro C.
通讯作者：
Amico, Sandro C.
影响因子：
4.9
作者：
Lou, Yu-Lun;Peng, Yu-Shiang;Leong, Kam W.
通讯作者：
Leong, Kam W.
影响因子：
4.7
作者：
Akinc, A;Anderson, DG;Langer, R
通讯作者：
Langer, R
影响因子：
3.4
作者：
Davis, HE;Rosinski, M;Yarmush, ML
通讯作者：
Yarmush, ML