ANTIBODY-DEPENDENT MURINE MACROPHAGE-MEDIATED DAMAGE TO SCHISTOSOMA-MANSONI SCHISTOSOMULA INVITRO
ANTIBODY-DEPENDENT MURINE MACROPHAGE-MEDIATED DAMAGE TO SCHISTOSOMA-MANSONI SCHISTOSOMULA INVITRO
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DOI：
10.1016/0008-8749(82)90025-9
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发表时间：
1982-01-01
影响因子：
4.3
通讯作者：
BRUCE, JI
中科院分区：
文献类型：
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作者：
CHUNG, PR;ASCH, HL;BRUCE, JI
抗体依赖性细胞介导的细胞毒性（ADCC）对人类寄生虫S。使用来自小鼠的抗血清和腹膜渗出细胞（PEC）证明了mansoni。PEC分为塑料粘附（96%巨噬细胞，4%淋巴细胞）和非粘附（92%淋巴细胞，8%巨噬细胞）细胞群。ADCC的四个标准，包括最小和最大的细胞附着，和死亡和台盼蓝摄取的青少年。使用正常小鼠的细胞和抗血清从巨噬细胞感染的小鼠，巨噬细胞粘附，破坏皮层和底层结构，并杀死童虫孵育18小时后观察。在同源系统中，当比较远交CD-1和近交BALB/c小鼠时，结果相似，除了来自后者小鼠的抗血清效力在感染后6 - 7周后降低，而对于前一品系小鼠则相反。非贴壁细胞也表现出ADCC对童虫，但效力大大低于贴壁细胞。这些补体非依赖性ADCC反应是阶段特异性的，因为成虫没有发生反应。
Antibody-dependent cell-mediated cytotoxicity (ADCC) against schistosomula of the human parasite S. mansoni was demonstrated using antisera from mice plus peritoneal exudate cells (PEC). PEC were divided into plastic-adherent (96% macrophages, 4% lymphocytes) and nonadherent (92% lymphocytes, 8% macrophages) cell populations. Four criteria of ADCC were used, including minimal and maximal cell attachment, and death of and uptake of trypan blue by schistosomula. Using cells from normal mice and antisera from schistosome-infected mice, macrophages adhered to, damaged the tegument and underlying structures of, and killed schistosomula when observed following 18 h incubation. In homologous systems, the results were similar when outbred CD-1 and inbred BALB/c mice were compared, except that potency of antisera from the latter mice decreased after 6-7 wk postinfection, whereas the opposite was true for the former strain of mice. Nonadherent cells also exhibited ADCC against schistosomula, but the potency was considerably lower than that of adherent cells. These complement-independent ADCC reactions were stage-specific for the schistosomulum in that no reactions occurred with adult worms.