Quantitative determination of apolipoproteins C-I and C-II in human plasma by separate electroimmunoassays.
Quantitative determination of apolipoproteins C-I and C-II in human plasma by separate electroimmunoassays.
复制标题
通过单独的电免疫测定法定量测定人血浆中的载脂蛋白 C-I 和 C-II。
DOI：
10.1093/clinchem/27.4.543
复制
发表时间：
1981
影响因子：
9.3
通讯作者：
P. Alaupovic
中科院分区：
文献类型：
--
作者：
M. D. Curry;W. Mcconathy;J. Fesmire;P. Alaupovic
描述了用于测量人血浆载脂蛋白C-I和C-II的单独电免疫测定法。纯化的载脂蛋白C-I和CII用于制备单特异性抗血清并作为初级标准品。这些检测方法灵敏度高，（最大灵敏度，20 ng），特异性，快速，精密（两种试验的试验内和试验间变异系数分别为5%和8%），（准确度基于计算和测量的C-I，C-II，和含ApoC的柱-洗脱液级分的C-III含量），并且适用于测量全血浆和密度类别中的C-I和C-II多肽。但是，甘油三酯（三酰甘油）浓度大于6000 mg/L的血浆样本必须在分析C-II前进行脱脂，浓度大于12000 mg/L的血浆样本也必须在分析C-I多肽前进行脱脂。血脂正常受试者和高脂蛋白血症表型IIa、IIb、IV和V受试者血浆中C-I的平均浓度（和SD）分别为60（15）、70（20）、100（20）、100（20）和260（94）mg/L。相应的C-II值分别为40（20）、43（20）、68（20）、65（20）和210（70）。表型IIb v、IV或V患者的C-I和C-II浓度显著（p <0.001）超过正常人或表型IIa。观察到的甘油三酯与C-I和C-II值之间的相关性（r = 0.92和r = 0.94）表明，这两种多肽，如C-III，是用于评估甘油三酯代谢状态的极好的血浆标志物。
Separate electroimmunoassays are described for measuring human plasma apolipoproteins C-I and C-II. Purified apolipoproteins C-I and CII were used in preparing monospecific antisera and as the primary standards. These assays are sensitive (maximal sensitivity, 20 ng), specific, rapid, precise (the within- and between-assay coefficients of variation for both assays were 5 and 8%, respectively), and accurate (accuracy was based on comparison of calculated and measured C-I, C-II, and C-III contents of an ApoC-containing column-eluent fraction) and are applicable to measurement of C-I and C-II polypeptides in whole plasma and density classes. However, plasma samples with triglyceride (triacylglycerol) concentrations greater than 6000 mg/L must be delipidized before analysis for C-II, as must those with greater than 12 000 mg/L before analysis for C-I polypeptide. Mean concentrations (and SD) of C-I in plasma of normolipidemic subjects and hyperlipoproteinemic phenotypes IIa, IIb, IV, and V were 60 (15), 70 (20), 100 (20), 100 (20), and 260 (94) mg/L, respectively. The corresponding C-II values were 40 (20), 43 (20), 68 (20), 65 (20), and 210 (70), respectively. C-I and C-II concentrations in patients with phenotypes IIb v, IV, or V significantly (p less than 0.001) exceeded those in normal persons or phenotype IIa. The observed correlations (r = 0.92 and r = 0.94) between triglyceride and C-I and C-II values suggest that these two polypeptides, like C-III, are excellent plasma markers for assessing the state of triglyceride metabolism.