The second transcribed spacer rDNA sequence: an effective genetic marker for inter∼species phylogenetic analysis of trematodes in the order Strigeata
The second transcribed spacer rDNA sequence: an effective genetic marker for inter∼species phylogenetic analysis of trematodes in the order Strigeata
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第二转录间隔区rDNA序列：白脚吸虫种间系统发育分析的有效遗传标记
DOI：
10.1007/s00436-012-2981-z
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发表时间：
2012-10-01
影响因子：
2
通讯作者：
Zhu, X. Q.
中科院分区：
文献类型：
--
作者：
Zhao, G. H.;Li, J.;Zhu, X. Q.
本研究对日本血吸虫中国大陆分离株的第二核内转录间隔区(ITS-2rDNA)进行了扩增、测序和分析，以推测吸虫在种内和种间的系统发育关系。从24S获得含有ITS-2 rDNA的片段。大陆8个流行省份的日本血吸虫分离株中国。ITS-2 rDNA序列在343~346个碱基之间存在长度多态，ITS-2序列的群体内和群体间变异在0.0%~2.1%之间。中国分离的日本血吸虫。用最大简约法和最大似然法进行的系统发育分析表明，ITS-2 rDNA序列不适合用于研究INS的种群间和种群内变异。日本产的。然而，对该目吸虫的系统发育分析表明，ITS-2 rDNA序列为研究该目吸虫的种间系统发育关系提供了一个有效的分子标记。
In the present study, the second nuclear internal transcribed spacer (ITS-2) rDNA ofSchistosoma japonicumisolates in mainland China was amplified, sequenced, and assessed for inferring the intra- and inter-species phylogenetic relationships of trematodes in the order Strigeata. The fragment containing ITS-2 rDNA was obtained from 24S. japonicumisolates from eight epidemic provinces in mainland China. The length polymorphisms were observed among these ITS-2 rDNA sequences, ranging from 343 to 346 bp, and the intra- and inter-population variations in ITS-2 sequence were 0.0–2.1 % amongS. japonicumisolates in China. Phylogenetic analyses using the maximum parsimony and maximum likelihood methods revealed that the ITS-2 rDNA sequence is not a suitable marker for studying inter- and intra-population variation inS. japonicum. However, phylogenetic analysis of trematodes in the order Strigeata indicated that the ITS-2 rDNA sequence provides an effective molecular marker for studying inter-species phylogenetic relationships among trematodes in this order.