A Ribose-Scavenging System Confers Colonization Fitness on the Human Gut Symbiont Bacteroides thetaiotaomicron in a Diet-Specific Manner
A Ribose-Scavenging System Confers Colonization Fitness on the Human Gut Symbiont Bacteroides thetaiotaomicron in a Diet-Specific Manner
复制标题
DOI：
10.1016/j.chom.2019.11.009
复制
发表时间：
2020-01-08
影响因子：
30.3
通讯作者：
Martens, Eric C.
中科院分区：
文献类型：
--
作者：
Glowacki, Robert W. P.;Pudlo, Nicholas A.;Martens, Eric C.
人类肠道中有效的营养获取对于微生物的持久性至关重要。虽然多糖已被充分研究为肠道微生物组的营养素，但其他资源如核酸和核苷的研究较少。我们描述了几个核糖利用系统（RUSs），广泛代表拟杆菌，似乎已经多样化，从各种底物中获取核糖。一种多形拟杆菌罗斯变体对于以饮食特异性方式竞争性肠道定殖至关重要。我们使用分子遗传学来探测系统所需的功能和这种表型背后的营养源的性质。两个RUS编码的核糖激酶是这种效应所需的唯一组分，大概是因为它们从未连接但必需的核苷磷酸化酶的产物中产生核糖磷酸衍生物。我们的研究结果强调了肠道共生体已经进化到获取营养物质的广泛机制，以及介导殖民化和持久性的系统之间意外依赖的可能性。
Efficient nutrient acquisition in the human gut is essential for microbial persistence. Although polysaccharides have been well-studied nutrients for the gut microbiome, other resources such as nucleic acids and nucleosides are less studied. We describe several ribose-utilization systems (RUSs) that are broadly represented in Bacteroidetes and appear to have diversified to access ribose from a variety of substrates. One Bacteroides thetaiotaomicron RUS variant is critical for competitive gut colonization in a diet-specific fashion. We used molecular genetics to probe the required functions of the system and the nature of the nutrient source(s) underlying this phenotype. Two RUS-encoded ribokinases were the only components required for this effect, presumably because they generate ribose-phosphate derivatives from products of an unlinked but essential nucleoside phosphorylase. Our results underscore the extensive mechanisms that gut symbionts have evolved to access nutrients and the potential for unexpected dependencies among systems that mediate colonization and persistence.