Photoinduced apoptosis using a peptide carrying a photosensitizer.
Photoinduced apoptosis using a peptide carrying a photosensitizer.
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使用携带光敏剂的肽进行光诱导细胞凋亡。
DOI：
10.1016/j.bmcl.2016.04.091
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发表时间：
2016
影响因子：
2.7
通讯作者：
T. Ohtsuki
中科院分区：
文献类型：
--
作者：
Kazunori Watanabe;Hayato Fujiwara;Mizuki Kitamatsu;T. Ohtsuki
合成了由HIV 1达特细胞穿透肽、Bim细胞凋亡诱导肽和Alexa Fluor 546组成的新型分子TatBim-Alexa，用于光诱导细胞凋亡。Alexa Fluor 546用作光敏剂，并通过巯基-马来酰亚胺反应共价连接在TatBim肽的C-末端。TatBim-Alexa的光依赖性胞质内化和使用TatBim-Alexa的光依赖性凋亡在包括人癌细胞系在内的多种哺乳动物细胞中得到证实。
A novel molecule, TatBim-Alexa, consisting of the HIV1 Tat cell-penetrating peptide, the Bim apoptosis-inducing peptide, and Alexa Fluor 546 was synthesized for photoinducion of apoptosis. The Alexa Fluor 546 was used as a photosensitizer and covalently attached at the C-terminus of TatBim peptide by the thiol–maleimide reaction. Photo-dependent cytosolic internalization of TatBim-Alexa and photo-dependent apoptosis using TatBim-Alexa were demonstrated in several kinds of mammalian cells including human cancer cell lines.
影响因子：
5.8
作者：
Chu, Rong;Terrano, David T.;Chambers, Timothy C.
通讯作者：
Chambers, Timothy C.