BMC Bioinformatics BioMed Central
BMC Bioinformatics BioMed Central
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DOI：
10.1007/bf00197814
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发表时间：
2008
影响因子：
2.7
通讯作者：
中科院分区：
文献类型：
--
作者：
在平面线圈探针中，在玻璃板之间的脂质双层中定向的15 N标记的爪蟾抗菌肽样品上获得了三维1H化学位移/1H-15 N偶极耦合/15 N化学位移相关光谱。光谱显示在所有三个维度上从两个标记的酰胺位点的共振的完全分辨率。与每个共振相关的三个取向依赖性频率使得能够相对于所施加的磁场的方向确定肽平面的取向。这些结果表明，多脉冲光谱的可行性，在一个平面线圈探头，在一个单一的实验中，从每个网站测量三个光谱参数的能力，并在定向膜蛋白的许多标记位点之间的解决潜力。
A three-dimensional1H chemical shift/1H-15N dipolar coupling/15N chemical shift correlation spectrum was obtained on a sample of specifically15N-labeled magainin peptides oriented in lipid bilayers between glass plates in a flat-coil probe. The spectrum showed complete resolution of the resonances from two labeled amide sites in all three dimensions. The three orientationally dependent frequencies associated with each resonance enabled the orientation of the peptide planes to be determined relative to the direction of the applied magnetic field. These results demonstrate the feasibility of multiple-pulse spectroscopy in a flat-coil probe, the ability to measure three spectral parameters from each site in a single experiment, and the potential for resolving among many labeled sites in oriented membrane proteins.