Inhibitory effect of the human liver-derived antimicrobial peptide hepcidin 20 on biofilms of polysaccharide intercellular adhesin (PIA)-positive and PIA-negative strains of Staphylococcus epidermidis
Inhibitory effect of the human liver-derived antimicrobial peptide hepcidin 20 on biofilms of polysaccharide intercellular adhesin (PIA)-positive and PIA-negative strains of Staphylococcus epidermidis
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DOI：
10.1080/08927014.2014.888062
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发表时间：
2014-04-21
期刊：
影响因子：
2.7
通讯作者：
Batoni, Giovanna
中科院分区：
文献类型：
--
作者：
Brancatisano, Franca Lisa;Maisetta, Giuseppantonio;Batoni, Giovanna
表皮葡萄球菌在生物膜相关医疗器械感染中起主要作用。本文中，针对S.表皮。Hep 20显着抑制生物膜的形成和细菌细胞代谢的PIA阳性和PIA阴性菌株，但生物膜生物量的减少，只有部分与活菌的减少。共聚焦显微镜图像显示，在hep 20的存在下，PIA阳性和PIA阴性菌株都形成了具有改变的结构和减少的细胞外基质量的生物膜。hep 20与万古霉素的共孵育在防止生物膜形成和处理预先形成的生物膜方面均不产生协同效应，以活细胞的数量来评价。相反，在hep 20存在下获得的生物膜，然后暴露于万古霉素，显示出增加的万古霉素敏感性。这些结果表明，hep 20可以抑制生物膜细胞外基质的产生/积累。
Staphylococcus epidermidis plays a major role in biofilm-related medical device infections. Herein the anti-biofilm activity of the human liver-derived antimicrobial peptide hepcidin 20 (hep20) was evaluated against polysaccharide intercellular adhesin (PIA)-positive and PIA-negative clinical isolates of S. epidermidis. Hep20 markedly inhibited biofilm formation and bacterial cell metabolism of PIA-positive and PIA-negative strains, but the decrease in biofilm biomass only partially correlated with a decrease in viable bacteria. Confocal microscope images revealed that, in the presence of hep20, both PIA-positive and PIA-negative strains formed biofilms with altered architectures and reduced amounts of extracellular matrix. Co-incubation of hep20 with vancomycin produced no synergistic effect, evaluated as number of viable cells, both in preventing biofilm formation and in treating preformed biofilms. In contrast, biofilms obtained in the presence of hep20, and then exposed to vancomycin, displayed an increased susceptibility to vancomycin. These results suggest that hep20 may inhibit the production/accumulation of biofilm extracellular matrix.