Juvenile hormone facilitates the antagonism between adult reproduction and diapause through the methoprene-tolerant gene in the female Colaphellus bowringi
Juvenile hormone facilitates the antagonism between adult reproduction and diapause through the methoprene-tolerant gene in the female Colaphellus bowringi
复制标题
保幼激素通过雌性 Colaphellus Bowringi 的耐甲氧普林基因促进成虫繁殖和滞育之间的拮抗作用
DOI：
10.1016/j.ibmb.2016.05.004
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发表时间：
2016-07-01
影响因子：
3.8
通讯作者：
Wang, Xiao-Ping
中科院分区：
文献类型：
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作者：
Liu, Wen;Li, Yi;Wang, Xiao-Ping
在昆虫中，保幼激素（JH）调节短日照（SD）诱导的生殖滞育的过程先前已被研究过。然而，我们仍然不了解 JH 调节长日照 (LD) 诱导的生殖滞育的机制。在本研究中，我们使用卷心菜甲虫Colaphellus Bowringi（一种亚洲十字花科蔬菜的严重害虫，能够在LD条件下进入生殖滞育）作为模型来测试JH是否调节雌性生殖滞育，其机制与SD诱导的滞育机制类似。我们的结果表明，JH 类似物 (JHA) 甲氧普林显着诱导卵巢发育，但抑制滞育成虫的脂质积累。同时，卵黄蛋白原（Vg）基因的转录本上调，而脂肪合成和应激耐受基因的表达下调。 JH 候选受体基因耐甲氧乙烯 (Met) 的 RNA 干扰可阻断 JH 诱导的卵巢发育和 Vg 转录，表明 JH-Met 信号在生殖中具有正向调节功能。此外，在繁殖诱导条件下，Met耗竭促进了滞育样表型，包括卵巢发育停滞和脂质储存增加，并刺激了参与脂质合成和应激耐受的滞育相关基因的表达，表明JH-Met信号在滞育抑制中发挥着重要作用。因此，我们的数据表明，JH 通过 Met 发挥作用，通过上调 Vg 表达来促进生殖系统的发育，同时通过抑制甘蓝甲虫的脂质合成和应激耐受性来抑制滞育。结合之前对 SD 诱导的生殖滞育的研究，我们得出结论，JH 可能通过保守的 Met 依赖性途径调节雌性生殖滞育，无论昆虫中诱导滞育的光周期长度如何。 (C) 2016 Elsevier Ltd. 保留所有权利。
In insects, the process whereby juvenile hormone (JH) regulates short-day (SD)-induced reproductive diapause has been previously investigated. However, we still do not understand the mechanism by which JH regulates long-day (LD)-induced reproductive diapause. In this study, we use a cabbage beetle, Colaphellus bowringi, which is a serious pest of cruciferous vegetables in Asia capable of entering reproductive diapause under LD conditions, as a model to test whether JH regulates female reproductive diapause similar to the mechanism of SD-induced diapause. Our results showed that the JH analog (JHA) methoprene significantly induced ovarian development but inhibited lipid accumulation of diapause-destined adults. Meanwhile, the transcripts of the vitellogenin (Vg) genes were upregulated, whereas the expression of the fat synthesis and stress tolerance genes were downregulated. RNA interference of the JH candidate receptor gene methoprene-tolerant (Met) blocked JH-induced ovarian development and Vg transcription, suggesting a positive regulatory function for JH-Met signaling in reproduction. Furthermore, under reproduction-inducing conditions, Met depletion promoted a diapause-like phenotype, including arrested ovarian development and increased lipid storage, and stimulated the expression of diapause-related genes involved in lipid synthesis and stress tolerance, suggesting JH-Met signaling plays an important role in the inhibition of diapause. Accordingly, our data indicate that JH acts through Met to facilitate development of the reproductive system by upregulating Vg expression while inhibiting diapause by suppressing lipid synthesis and stress tolerance in the cabbage beetle. Combined with previous studies in SD-induced reproductive diapause, we conclude that JH may regulate female reproductive diapause using a conserved Met-dependent pathway, regardless of the length of the photoperiod inducing diapause in insects. (C) 2016 Elsevier Ltd. All rights reserved.