ROLE OF MOTILITY IN APPLE BLOSSOM INFECTION BY ERWINIA-AMYLOVORA AND STUDIES OF FIRE BLIGHT CONTROL WITH ATTRACTANT AND REPELLENT COMPOUNDS

ROLE OF MOTILITY IN APPLE BLOSSOM INFECTION BY ERWINIA-AMYLOVORA AND STUDIES OF FIRE BLIGHT CONTROL WITH ATTRACTANT AND REPELLENT COMPOUNDS
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DOI:
10.1094/phyto-76-441
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发表时间:
1986-04-01
期刊:
影响因子:
3.2
通讯作者:
RIES, SM
RIES, SM
中科院分区:
农林科学2区
文献类型:
--
作者:
BAYOT, RG;RIES, SM

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用0.3M甲磺酸乙酯处理野生型梨火疫病菌(Erwiniamylovora)细胞,获得一株不运动的菌株(Nm 22)及其运动回复突变株(Mr 22)。这两个菌株的致病性相似的年轻乔纳森苹果(苹果)幼苗的野生型。在接种物浓度为5 × 105时,在用Mr 22接种的花中记录到比用Nm 22接种的花显著更多的感染。105和1 ×每毫升107个菌落形成单位无论是驱避剂,苯甲酸钠或水杨酸钠,也不是引诱剂,苹果酸钠或酒石酸钠,适用于苹果花作为10-2或10-5 M的解决方案,提供一致的保护,防止感染E。食淀粉的。E. amylovora是苯甲酸盐和水杨酸盐的组成型,但对L-异亮氨酸、L-亮氨酸和L-苯丙氨酸是诱导型。
A nonmotile strain (Nm22) and its motile revertant (Mr22) of Erwinia amylovora were obtained after treating cells of the wild type with 0.3 M ethyl methanesulfonate. Pathogenicity of both strains was similar to that of the wild type on young Jonathan apple (Malus domestica) seedlings. Significantly more infection was recorded in blossoms inoculated with Mr22 than with Nm22 at inoculum concentrations of 5 .times. 105 and 1 .times. 107 colony-forming units per milliliter. Neither the repellents, sodium benzoate or sodium salicylate, nor the attractants, sodium malate or sodium tartrate, applied to apple blossoms as 10-2 or 10-5 M solutions, provided consistent protection against infection by E. amylovora. Negative chemotaxis of E. amylovora was constitutive for benzoate and salicylate but inducible for L-isoleucine, L-leucine, and L-phenylalanine.