Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen.
Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen.
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DOI：
10.1084/jem.141.6.1376
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发表时间：
1975-06-01
期刊：
影响因子：
--
通讯作者：
Boyse EA
中科院分区：
文献类型：
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作者：
Cantor H;Boyse EA
由两个互不连锁的基因座(Ly-1和Ly-2/Ly-3)编码的Ly同种异体抗原在胸腺依赖分化的淋巴细胞上表达。根据Ly-1、Ly-2和Ly-3的表达差异，C57BL/6小鼠外周血中Thy-1+细胞可分为三个亚类，约50%表达三种Ly抗原(Ly-123+)，约33%仅表达Ly-1(Ly-1+)，约6-8%表达Ly-2和Ly-3(Ly-23+)。Ly-123+亚类细胞是个体发育中最早出现的外周Thy-1+细胞，成人胸腺切除后不久外周Thy-1+细胞减少。相反，Ly-1+和Ly-23+亚类在外周组织中出现的时间比Ly-123+细胞晚，并且对Ly-1+细胞和Ly-123+细胞(从而富含Ly-23+细胞)的成人胸腺淋巴淋巴群体的早期效应具有抵抗力，它们不能对SRBC产生显著的辅助活性，但对同种异体靶细胞产生大量的细胞毒活性。同样的淋巴群体，耗尽了Ly-23+细胞和Ly-123+细胞(从而丰富了Ly-1+细胞)，产生了大量的辅助反应，但无法产生明显的杀伤活性。这些实验表明，T细胞承诺只参与辅助功能或细胞毒功能是一个分化过程，发生在它们遇到抗原之前，并伴随着不同的Ly基团，分别是Lu-23或Ly-1，与TL+Ly-123+T细胞前体排斥。Ly-123+亚类的TL期是一种过渡型亚类，还是具有离散免疫功能的单独分化亚类，目前尚不清楚。
Ly alloantigens coded by two unlinked genetic loci (Ly-1 and Ly-2/Ly-3) are expressed on lymphoid cells undergoing thymus-dependent differentiation. Peripheral Thy-1+ cells from C57BL/6 mice can be divided into three subclasses on the basis of differential expression of Ly-1, Ly-2, and Ly-3; about 50% express all three Ly antigens (Ly - 123+), about 33% only Ly-1 (Ly-1+), and about 6-8% Ly-2 and Ly-3 (Ly- 23+). Cells of the Ly-123+ subclasses are the first peripheral Thy-1+ cells to appear in ontogeny, and are reduced in the periphery shortly after adult thymectomy. In contrast, Ly-1+ and Ly-23+ subclasses appear later in the peripheral tissues than do Ly-123+ cells, and are resistant to the early effects of adult thymectomymperiheral lymphoid populations depleted of Ly-1+ cells and Ly-123+ cells (and thereby enriched for Ly-23+ cells) were incapable of developing significant helper activity to SRBC but generated substantial levels of cytotoxic activity to allogeneic target cells. The same lymphoid populations, depleted of Ly-23+ cells and Ly-123+ cells (and thereby enriched for Ly- 1+ cells), produced substantial helper responses but were unable to generate appreciable levels of killer activity. These experiments imply that commitment of T cells to participate exclusively in either helper or cytotoxic function is a differentiative process that takes place before they encounter antigen, and is accompanied by exclusion of different Ly groups, Lu-23 or Ly-1 respectively, from TL+Ly-123+ T-cell precursors. It is yet to be decided whether the TL-phase by Ly-123+ subclass is a transitional form or a separately differentiated subclass with a discrete immunologic function.