PHYTOHEMAGGLUTININS - A NEW CLASS OF METALLOPROTEINS - ISOLATION, PURIFICATION, AND SOME PROPERTIES OF LECTIN FROM PHASEOLUS-LUNATUS
PHYTOHEMAGGLUTININS - A NEW CLASS OF METALLOPROTEINS - ISOLATION, PURIFICATION, AND SOME PROPERTIES OF LECTIN FROM PHASEOLUS-LUNATUS
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DOI：
10.1016/0014-5793(70)80354-4
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发表时间：
1970-01-01
期刊：
影响因子：
3.5
通讯作者：
GOLDSTEIN, IJ
中科院分区：
文献类型：
--
作者：
GALBRAITH, W;GOLDSTEIN, IJ
植物血凝素是广泛存在于豆科植物种子中的碳水化合物结合球蛋白[1，2]。这些种子蛋白为研究同质结合位点提供了一个独特的机会，这一可能性尚未通过抗体实现。一些凝集素的特性已被详细研究[3-61]。刀豆凝集素A(ConA)是菜豆(Canavalia Ensfomis)的植物血凝素，是唯一一种纯化的不是糖蛋白的凝集素，其金属需求已确定[7-91]。关于其他植物血凝素的金属需求还没有报道，尽管已经提到乙二胺四乙酸酯(EDTA)取消了普通菜豆(Pi-IA)的凝集和促有丝分裂的特性，PI-IA是菜豆的粗提物，被不同地称为蜡豆或菜豆[Lo，II]。利马豆的凝集素以其粗提物或部分纯化的制剂的形式进行了广泛的研究[12-141]，已被证明是对A型人红细胞的特异性的，并被N-乙酰-D-半乳糖胺(GalNAc)特异性抑制。最近，Gould和Scheinberg[151]公布了利马豆植物血凝素的分离程序，并报告了它的一些物理和化学性质。发现了两种活性成分--其中一种的分子量是另一种的两倍。
Phytohemagglutinins(lectins) are carbohydratebinding globulin proteins found in the seeds of a wide variety of leguminous plants [1, 2]. These seed proteins provide a unique opportunity for the investigation of homogeneous combining sites, a possibility not yet realized with antibodies. The specificities of a few lectins have been studied in some detail [3-61. Concanavalin A (con A) the phytohemagglutinin of the jack bean (Canavalia ensifomis) is the only purified lectin which is not a glycoprotein and for which a metal requirement has been established conclusively [7-91. There have been no reports concerning the metal requirements of other phytohemagglutinins, although reference has been made to the fact that ethylenediaminetetraacetate(EDTA) abolishes the agglutinating and mitogenic stimulating properties of PI-IA, a crude extract of Phaseolus vulgaris, variously referred to as wax bean or kidney bean [lo, II].The lectin of the lima bean, Phaseolus lunatus, studied extensively in the form of its crude extracts or partially purified preparations [12-141, has been shown to be specific for type A human red blood cells and to be specifically inhibited by N-acetyl-D-galactosamine (GalNAc). Recently, Gould and Scheinberg [151 published an isolation procedure for the lima bean phytohemagglutinin and reported some of its physical and chemical properties. Two active components were found-one with twice the molecular weight of the other.