Expression of pregnancy-specific beta 1-glycoprotein genes in hematopoietic cells.
Expression of pregnancy-specific beta 1-glycoprotein genes in hematopoietic cells.
复制标题
妊娠特异性 β1-糖蛋白基因在造血细胞中的表达。
DOI：
10.1007/bf01076099
复制
发表时间：
1993
影响因子：
4.3
通讯作者：
Chan,WY
中科院分区：
文献类型：
--
作者：
Wu,SM;Bazar,LS;Cohn,ML;Cahill,RA;Chan,WY
免疫组织化学染色证实PSG存在于血细胞中。然而，这些蛋白质的来源尚不清楚。这份报告检测了PSG基因在不同类型的新鲜分离的血细胞中的表达。从健康人骨髓和外周血细胞中提取RNA，利用合成的针对PSG基因的寡核苷酸引物，通过逆转录聚合酶链式反应分析PSG转录本。PSG基因在不同类型细胞中的表达水平表现出显著的个体差异。在中性粒细胞(PMN)、单核细胞和B淋巴细胞中均可检测到微量PSG转录本，而T淋巴细胞转录本含量最高。PSG基因在血细胞中的表达明显不受分离方法和过夜培养的影响，但用绵羊红细胞玫瑰花环分离淋巴细胞的情况除外。所有报道的PSG转录本都在血细胞中检测到。除PSG5和PSG11的II型转录本仅在胎盘中发现外，在血细胞中均检测到PSG基因的I型和II型转录本。一些PSG家族成员的表达或选择性剪接涉及组织特异性。
The presence of PSG in blood cells has been demonstrated by immunohistochemical staining. However, the origin of these proteins is not known. This report examines the expression of the PSG genes in different types of freshly isolated blood cells. RNA isolated from bone marrow and peripheral blood cells of healthy individuals was analyzed for PSG transcripts by reverse transcriptase-polymerase chain reaction using synthetic oligonucleotide primers specific for the PSG genes. The level of expression of the PSG genes in different types of cells exhibited significant individual variation. Trace amounts of PSG transcripts could be detected in polymorphonuclear cells (PMN), monocytes and B lymphocytes while T lymphocytes always contained the highest level of transcript. The expression of PSG genes in the blood cells apparently was not affected by the method of isolation nor by overnight culturing of these cells except in the case when lymphocytes were separated by rosetting with sheep red blood cells. All reported PSG transcripts were detected in blood cells. Both type I and type II transcripts of the PSG genes were detected in blood cells with the exception of type II transcript of PSG5 and PSG11 which were only found in the placenta. Tissue specificity in the expression or alternative splicing of some of the PSG family members was implicated.