Fine structure of the luminous spines and luciferase detection in the brittle star Amphiura filiformis
Fine structure of the luminous spines and luciferase detection in the brittle star Amphiura filiformis
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DOI：
10.1016/j.jcz.2017.05.001
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发表时间：
2017-01-01
影响因子：
1.4
通讯作者：
Mallefet, Jerome
中科院分区：
文献类型：
--
作者：
Delroisse, Jerome;Ullrich-Lueter, Esther;Mallefet, Jerome
丝状Amphiura filformis是一种常见的穴居海蛇尾，生活在欧洲海岸泥泞的环境中。当受到机械刺激时，该物种会在手臂脊柱尖端发出蓝光。虽然最近发现了丝状棘虫的荧光素酶，但该物种以及大多数其他生物发光棘皮动物的发光细胞（即光细胞）的超微结构仍然未知。在这项研究中，我们描述了海蛇尾A. filiformis的臂和棘的形态，并将其与免疫组织化学显示的荧光素酶表达模式进行了比较。荧光素酶在脊柱底部和臂尖的明确的光细胞簇内表达。此外，我们还研究了光化学刺激前后脊椎组织的超微结构，以鉴定光细胞，提高我们对海蛇尾光发生现象的认识。II型颗粒细胞位于脊柱内部组织的基底到中基底部分，在发光过程中表现出超微结构的改变，被认为是光细胞。推测的光细胞似乎与脊神经丛以及粘液细胞和推测的色素细胞密切相关。光细胞的特征是在其细胞质中存在具有网状内容物的特定囊泡。此外，使用显微ct扫描研究了钙化脊柱小骨的结构。(C) 2017 Elsevier GmbH版权所有。
Amphiura filiformis is a common burrowing brittle star from muddy environments along the European shores. This species emits a blue light at the level of the arm spine tips when mechanically stimulated. Although the putative luciferase of A. filiformis was recently discovered, the ultrastructure of the luminous cells, i.e. the photocytes, remain unknown for this species as well as for most other bioluminescent echinoderms. In this study, we describe the morphology of the arm and spines in the brittle star A. filiformis and compare it to the luciferase expression pattern revealed by immunohistochemistry. Luciferase is expressed at the base of the spine and at the arm tips within well-defined photocyte clusters. Additionally, we investigated the ultrastructure of spine tissues before and after chemical stimulation of light emission in order to identify photocytes and improve our comprehension of the photogenesis phenomenon in brittle stars. Type II granular cells, found in the basal to mid-basal portion of the spine inner tissues and showing ultrastructural modifications during light-emission, are proposed as photocytes. Putative photocytes appear intimately associated with the spine nerve plexus as well as with mucus cells and presumptive pigment cells. Photocytes are characterised by the presence of specific vesicles with reticulated contents in their cytoplasm. Furthermore, the structure of the calcified spine ossicle has been investigated using microCT scanning. (C) 2017 Elsevier GmbH. All rights reserved.