Single-Molecule Detection of DNA-Stabilized Silver Nanoclusters Emitting at the NIR I/II Border
Single-Molecule Detection of DNA-Stabilized Silver Nanoclusters Emitting at the NIR I/II Border
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DOI：
10.1021/acs.jpclett.0c03688
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发表时间：
2021-01-21
影响因子：
5.7
通讯作者：
Vosch, Tom
中科院分区：
文献类型：
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作者：
Liisberg, Mikkel B.;Kardar, Zahra Shakeri;Vosch, Tom
已知近红外（NIR）I和II区域具有良好的组织透光性，并且与电磁光谱的可见光区域相比具有较少的散射。然而，这些区域中明亮荧光团的数量是有限的。在这里，我们提出了一个详细的光谱表征的DNA稳定的银纳米团簇（DNA-AgNC），在960 nm左右的溶液中发射。DNA-AgNC随时间转化为蓝移发射体。将这些DNA-AgNC包埋在聚乙烯醇（PVA）中表明，它们具有足够的亮度和光稳定性，可以在单分子水平上检测到。进行光子反聚束实验以确认单发射极行为。我们的研究结果强调，在NIR II区域中筛选和探索DNA-AgNC 5可能会产生有前途的明亮，光稳定的发射体，可以帮助开发具有前所未有的信号背景比和单分子灵敏度的生物成像应用。
The near-infrared (NIR) I and II regions are known for having good light transparency of tissue and less scatter compared to the visible region of the electromagnetic spectrum. However, the number of bright fluorophores in these regions is limited. Here we present a detailed spectroscopic characterization of a DNA-stabilized silver nanocluster (DNA-AgNC) that emits at around 960 nm in solution. The DNA-AgNC converts to blueshifted emitters over time. Embedding these DNA-AgNCs in poly(vinyl alcohol) (PVA) shows that they are bright and photostable enough to be detected at the single-molecule level. Photon antibunching experiments were performed to confirm single emitter behavior. Our findings highlight that the screening and exploration of DNA-AgNC5 in the NIR II region might yield promising bright, photostable emitters that could help develop bioimaging applications with unprecedented signal-to-background ratios and single-molecule sensitivity.