Osteoblast differentiation onto different biometals with an endoprosthetic surface topography in vitro
Osteoblast differentiation onto different biometals with an endoprosthetic surface topography in vitro
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DOI：
10.1002/jbm.a.31552
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发表时间：
2008-07-01
影响因子：
4.9
通讯作者：
Krauspe, R.
中科院分区：
文献类型：
--
作者：
Jaeger, M.;Urselmann, F.;Krauspe, R.
在这项体外研究中，我们比较了三种不同合金（手术钢、CoCr、Ti6Al4V）和三种不同表面结构（抛光、喷砂、多孔涂层）对人骨髓细胞培养物的细胞相容性和成骨细胞促进效力。这些生物金属之所以被特别选择，是因为它们目前在临床骨科实践中的应用。将人单核骨髓细胞培养在不同生物材料的表面上，并用地塞米松、L-抗坏血酸-2-磷酸盐和β-甘油磷酸盐刺激3周。针对几种抗原（ALP、RANKL、骨桥蛋白、胶原蛋白1）的免疫荧光染色、胶原蛋白（Col）I/II、BSP、骨桥蛋白、骨钙蛋白、TRAP、光学和扫描电子显微镜评估的mRNA表达来评估细胞生长和成骨细胞分化。对于样品的表面粗糙度和能量分析，进行了粗糙度轮廓（Ra、Rz）和接触角（CA）测量。我们发现不同生物金属和表面结构之间存在差异。钢显示出潜在的细胞毒性作用，而 CoCr 和更多 Ti6Al4V 显示出优异的细胞相容性。每种测试的生物材料之间的 mRNA 表达没有质量差异。在抗原表达方面，喷砂 Ti6Al4V 表面显示出增强的成骨细胞分化。与抛光表面相比，多孔涂层表面提高了 CoCr 的骨传导性。与对照相比，用生物金属培养的所有细胞均诱导 RANKL 表达。除喷砂 Ti6Al4V 外，细胞增加了种植体的粗糙度。我们的数据表明，生物金属的表面形貌和理化性质影响体外成骨细胞分化。 (C) 2007 年 Wiley 期刊公司。
In this in vitro study, we compared the cytocompatibility and osteoblast promoting potency on human bone marrow cell culture with three different alloys (surgical steel, CoCr, Ti6Al4V) and three different surface structures (polished, sandblasted, porous coated). These biometals were specifically chosen because of their current applications in clinical orthopedic practices. Human mononuclear bone marrow cells were cultivated onto the surface of the different biomaterials and stimulated by dexamethasone, L-ascorbic-acid-2-phoshpate and beta-glycerolphosphate over a 3-week period. Immunofluorescent stainings against several antigens (ALP, RANKL, osteopontin, collagen 1), mRNA-expression of collagen (Col) I/II, BSP, osteopontin, osteocalcin, TRAP, light and scanning electron microscopy evaluation were used to evaluate cellular growth and osteoblast differentiation. For surface roughness and energy analysis of the specimen, roughness profile (Ra, Rz) and contact angle (CA) measurements were performed. We found differences between the different biometals and surface structures. Steel showed potential cytotoxic effects whereas CoCr and more Ti6Al4V showed an excellent cytocompatibility. There were no qualitative differences in mRNA expression between each of the tested biomaterials. In terms of antigen expression, a sandblasted Ti6Al4V surface showed enhanced osteoblastic differentiation. A porous-coated surface improved the osteoconductivity of CoCr when compared to a polished surface. In contrast to controls all cells cultivated with biometals induced a RANKL expression. Cells increased the implant roughness with the exception of sandblasted Ti6Al4V. Our data show that surface topography and physicochemical properties of biometals influence osteoblast differentiation in vitro. (C) 2007 Wiley Periodicals, Inc.