Sensitive voltammetric determination of tryptophan using an acetylene black paste electrode modified with a Schiff's base derivative of chitosan
Sensitive voltammetric determination of tryptophan using an acetylene black paste electrode modified with a Schiff's base derivative of chitosan
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使用壳聚糖希夫碱衍生物修饰的乙炔黑糊电极灵敏伏安法测定色氨酸
DOI：
10.1039/c1an15351j
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发表时间：
2011-01-01
期刊：
影响因子：
4.2
通讯作者：
Feng, Yonglan
中科院分区：
文献类型：
--
作者：
Deng, Peihong;Fei, Junjie;Feng, Yonglan
采用水杨醛对壳聚糖进行席夫碱修饰，并采用滴涂法将其修饰在乙炔黑糊电极表面。在0.5 mol L ~（-1）醋酸盐缓冲溶液（pH 4.2）中，色氨酸（Trp）的阳极溶出峰电流（与常规裸碳糊电极（CPE）和裸ABPE相比）在Schiff碱壳聚糖修饰电极上有显著增加。对影响色氨酸伏安法测定的参数进行了优化。在选定的条件下，阳极峰电流与色氨酸浓度之间的线性范围为6.0 × 10（-8）mol L-1至2.0 × 10（-6）mol L-1，在2.0 × 10（-6）mol L-1 ~ 4.0 × 10（-5）mol L-1和4.0 × 10（-5）mol L-1 ~ 1.0 × 10（-4）mol L-1范围内，富集60 s后，检测下限为2.0 × 10（-9）mol L-1。该电化学传感器已成功地应用于药物和生物样品中色氨酸的测定，结果令人满意。
Chitosan was modified by salicylaldehyde via Schiff's base reaction and the resulting product was modified on the surface of an acetylene black paste electrode (ABPE) by the drop-coating method. In 0.5 mol L-1 acetate buffer (pH 4.2), a substantial increase in the anodic stripping peak current of tryptophan (Trp) (compared to conventional bare carbon paste electrode (CPE) and bare ABPE) is observed at the Schiff's base chitosan-modified electrode. The parameters influencing voltammetric determination of Trp have been optimized. Under the selected conditions, the linearity between the anodic peak currents and concentrations of Trp demonstrated a wide range of 6.0 x 10(-8) mol L-1 to 2.0 x 10(-6) mol L-1, 2.0 x 10(-6) mol L-1 to 4.0 x 10(-5) mol L-1 and 4.0 x 10(-5) mol L-1 to 1.0 x 10(-4) mol L-1, a low detection limit of 2.0 x 10(-9) mol L-1 was obtained after a 60s accumulation. In addition, the developed electrochemical sensor has been successfully applied for the determination of Trp in pharmaceutical and biological samples with satisfactory assay results.