Characterization of gut microbiota composition and functions in patients with chronic alcohol overconsumption
Characterization of gut microbiota composition and functions in patients with chronic alcohol overconsumption
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DOI：
10.1080/19490976.2019.1580097
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发表时间：
2019-11-02
期刊：
影响因子：
12.2
通讯作者：
Valeur, Jorgen
中科院分区：
文献类型：
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作者：
Bjorkhaug, Steinar Traae;Aanes, Havard;Valeur, Jorgen
过量的酒精摄入会改变肠道微生物群，这可能是酒精相关疾病的病理生理学基础。我们检查了饮酒过度超过10年的患者的肠道微生物群组成和功能，并与无酒精或低酒精摄入史的对照组患者进行了比较。通过16S rRNA测序评估粪便微生物群组成。通过定量短链脂肪酸（SCFA）和预测宏基因组分析（PICRUSt）来评估肠道微生物群功能。24例患者，平均年龄64.8岁（19例男性），饮酒过度，18例对照患者，平均年龄58.2岁（14例男性）。两组在基本临床变量方面相当。营养评估显示，在筛选工具迷你营养评估，较低的肌肉质量评估的握力，和较低的血浆维生素C水平在酒精过度消费组的总评分较低。变形菌门的细菌相对丰度较高，而粪杆菌属的细菌相对丰度较低的酒精过度消费者组。该组还具有较高水平的Sutterella，Holdemania和Clostridium属，以及较低的丁酸浓度和百分比。当应用PICRUSt预测宏基因组组成时，我们发现与上皮细胞侵袭相关的基因在酒精过度消费者中更常见。我们的结论是，酒精过度消费患者的肠道微生物群组成和功能与酒精消费量低的患者不同，并且似乎偏向于假定的促炎方向。
Excessive alcohol intake can alter the gut microbiota, which may underlie the pathophysiology of alcohol-related diseases. We examined gut microbiota composition and functions in patients with alcohol overconsumption for >10 years, compared to a control group of patients with a history of no or low alcohol intake. Faecal microbiota composition was assessed by 16S rRNA sequencing. Gut microbiota functions were evaluated by quantification of short-chain fatty acids (SCFAs) and predictive metagenome profiling (PICRUSt). Twenty-four patients, mean age 64.8 years (19 males), with alcohol overconsumption, and 18 control patients, mean age 58.2 years (14 males) were included. The two groups were comparable regarding basic clinical variables. Nutritional assessment revealed lower total score on the screening tool Mini Nutritional Assessment, lower muscle mass as assessed by handgrip strength, and lower plasma vitamin C levels in the alcohol overconsumption group. Bacteria from phylum Proteobacteria were found in higher relative abundance, while bacteria from genus Faecalibacterium were found in lower relative abundance in the group of alcohol overconsumers. The group also had higher levels of the genera Sutterella, Holdemania and Clostridium, and lower concentration and percentage of butyric acid. When applying PICRUSt to predict the metagenomic composition, we found that genes related to invasion of epithelial cells were more common in the group of alcohol overconsumers. We conclude that gut microbiota composition and functions in patients with alcohol overconsumption differ from patients with low consumption of alcohol, and seem to be skewed into a putative pro-inflammatory direction.