Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins
Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins
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DOI：
10.1038/nmeth.1479
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发表时间：
2010-08-01
期刊：
影响因子：
48
通讯作者：
Knoepfel, Thomas
中科院分区：
文献类型：
--
作者：
Akemann, Walther;Mutoh, Hiroki;Knoepfel, Thomas
皮质信息处理依赖于具有不同电生理和连接特性的不同类别神经元之间的突触相互作用。要揭示这些复杂电路的工作原理，需要探测特定神经元群体的电活动。在这里，我们表明，基因编码的电压敏感荧光蛋白（VSFP）提供了一个光学电压报告，从目标神经元在文化，急性脑切片和活小鼠。通过在小鼠躯体感觉皮层的锥体细胞中表达VSFP，我们还证明了这些探针可以报告对体内单一感觉刺激的皮层电反应。这些基于蛋白质的电压探针将有助于分析遗传定义的细胞群中的皮层电路，因此是光遗传学工具箱的一个有价值的补充。
Cortical information processing relies on synaptic interactions between diverse classes of neurons with distinct electrophysiological and connection properties. Uncovering the operational principles of these elaborate circuits requires the probing of electrical activity from selected populations of defined neurons. Here we show that genetically encoded voltage-sensitive fluorescent proteins (VSFPs) provide an optical voltage report from targeted neurons in culture, acute brain slices and living mice. By expressing VSFPs in pyramidal cells of mouse somatosensory cortex, we also demonstrate that these probes can report cortical electrical responses to single sensory stimuli in vivo. These protein-based voltage probes will facilitate the analysis of cortical circuits in genetically defined cell populations and are hence a valuable addition to the optogenetic toolbox.