Hock immunization: A humane alternative to mouse footpad injections
Hock immunization: A humane alternative to mouse footpad injections
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DOI：
10.1016/j.jim.2007.08.004
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发表时间：
2007-12-01
影响因子：
2.2
通讯作者：
Kamala, T.
中科院分区：
文献类型：
--
作者：
Kamala, T.
足垫注射是小鼠常用的免疫方法。由于相对容易进行良好的特征性淋巴引流，它已成为一个非常有用的免疫方案，以研究在引流淋巴结的局部免疫反应。然而，其缺点包括仅使用后足作为常规免疫部位，因为小鼠使用其前足处理食物，以及注射部位的炎症和肿胀加剧，导致未缓解的疼痛和痛苦，因为脚是承重结构。随着越来越严格的动物操作机构指南，越来越需要更人道的协议。一种新的免疫方案，包括注射到跗关节，踝关节上方的外侧跗骨区域，一个非承重结构，引流到与脚垫相同的淋巴结，保留了脚垫免疫的优点，而没有其缺点。本研究比较了六种不同近交系小鼠对两种不同蛋白质抗原和热灭活细菌的足垫和飞节免疫之间的免疫反应，表明飞节免疫是足垫免疫的更好替代方案，诱导可比的免疫反应，并且更加人性化。(C)2007 Elsevier B.V.保留所有权利。
Footpad injection is a commonly used immunization method in mice. Being relatively easy to do with well-characterized lymphatic drainage, it has become a very useful immunization protocol to study local immune responses in draining lymph nodes. However, its disadvantages include use of only hind feet as a routine site of immunization since mice use their fore feet for food handling, and exacerbation of inflammation and swelling at the injection site leading to unrelieved pain and distress since feet are weight-bearing structures. With increasingly stringent Institutional guidelines for animal manipulations, there is increasing need for more humane protocols. A novel immunization protocol involving injection into the hock, the lateral tarsal region just above the ankle, a non-weight bearing structure draining to the same lymph node as the footpad, retains the advantages of footpad immunization without its drawbacks. This study, comparing immune responses between footpad and hock immunization in six different inbred mouse strains to two different protein antigens and a heat-killed bacterium, shows that hock immunization is a better alternative to footpad immunization, inducing comparable immune responses and being considerably more humane. (C) 2007 Elsevier B.V. All rights reserved.