Fe-T1 Sensor Based on Coordination Chemistry for Sensitive and Versatile Bioanalysis
Fe-T1 Sensor Based on Coordination Chemistry for Sensitive and Versatile Bioanalysis
复制标题
基于配位化学的 Fe-T-1 传感器，用于灵敏且多功能的生物分析
DOI：
10.1021/acs.analchem.8b01577
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发表时间：
2018-08-07
影响因子：
7.4
通讯作者：
Jiang, Xingyu
中科院分区：
文献类型：
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作者：
Dong, Mingling;Zheng, Wenshu;Jiang, Xingyu
顺磁离子介导的磁传感器的主要挑战是其相对较低的灵敏度。在本研究中，我们观察到Fe2+转变为Fe3+并与硫氰酸钾（KSCN）配位时纵向弛豫时间（T-1）信号的放大，并基于KSCN和Fe-3（+）之间的配位化学开发了一种灵敏的Fe-T-1传感器，以放大T-1信号以检测一系列目标物，例如过氧化氢、葡萄糖和 抗原/抗体。我们通过成功检测牛奶样品中的四环素和临床样品中的丙型肝炎病毒且准确度令人满意，证明了我们的测定的实用性。这种KSCN介导的Fe-T-1传感器不仅实现了更高灵敏度的生化分析和免疫分析，而且保留了顺磁离子介导的磁传感器的许多优点（稳定性好、操作简单），对于复杂样品中一系列感兴趣目标的检测具有广阔的前景。
The main challenge of paramagnetic ions-mediated magnetic sensors is their relatively low sensitivity. In this study, we observe the amplification of longitudinal relaxation time (T-1) signal when Fe2+ transforms into Fe3+ followed by the coordination of potassium thiocyanate (KSCN) and develop a sensitive Fe-T-1 sensor based on the coordination chemistry between KSCN and Fe-3(+) to amplify the T-1 signal for detecting a series of targets, such as hydrogen peroxide, glucose, and antigen/antibody. We justify the practicability of our assay by successfully detecting tetracycline in milk samples and hepatitis C virus in clinical samples with satisfactory accuracy. This KSCN-mediated Fe-T-1 sensor not only realizes biochemical analysis and immunoassay with higher sensitivity but also retains many advantages of paramagnetic ions-mediated magnetic sensors (good stability and straightforward operation), which holds great promise for the detection of a range of targets of interest in complex samples.