Tandem repetition of baculovirus ie1 promoter results in upregulation of transcription
Tandem repetition of baculovirus ie1 promoter results in upregulation of transcription
复制标题
杆状病毒 ie1 启动子的串联重复导致转录上调
作者：
Katsura Kojima;Tohru Hayakawa;S. Asano;H. Bando
概括。 为了改进昆虫细胞中不依赖于病毒的基因表达系统，研究了杆状病毒立即早期基因BmNPV ie1的启动子序列的重复对基因表达活性的影响。 ie1 启动子的串联重复导致报告基因表达活性增加约 4 倍。引物延伸分析表明，重复不仅增加了转录本的数量（4至5倍），而且还增加了转录起始位点的数量（至少3个额外的起始位点）。突变实验证明独立的顺式作用元件参与这两种不同的启动子增强作用。
Summary. In order to improve a virus-independent gene expression system in insect cells, the effect of the repetition of the promoter sequence of a baculovirus immediate early gene, BmNPV ie1, on the gene expression activity was examined. Tandem repetition of ie1 promoter resulted in about a 4-fold increase of the reporter gene expression activity. Primer extension analysis revealed that the repetition increased not only the amount of transcripts (4 to 5-times) but also the number of sites of transcription initiation (at least 3 extra initiation sites). A mutational experiment demonstrated the involvement of independent cis-acting elements in these two different promoter-enhancing effects.
转录因子 (TF) IIB 和 TFIIA 可以独立增加 TATA 结合蛋白对 DNA 的亲和力。
DOI：
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发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Imbalzano,AN;Zaret,KS;Kingston,RE
通讯作者：
Kingston,RE