Dynamic gut microbiome changes following regional intestinal lymphatic obstruction in primates.
Dynamic gut microbiome changes following regional intestinal lymphatic obstruction in primates.
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DOI：
10.1016/j.pathophys.2019.06.004
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发表时间：
2019-09-01
期刊：
影响因子：
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通讯作者：
Alexander, J S
中科院分区：
文献类型：
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作者：
Becker, F;Gavins, F N E;Alexander, J S
炎症性肠病 (IBD) 的发病机制与淋巴淤滞有关，但在克罗恩病 (CD) 的情况下，淋巴阻塞是否以及如何扰乱肠道微生物组目前尚不清楚。我们在非洲绿猴中采用了一种新的 CD 模型，称为“ATLAS”（非洲绿猴淋巴管阻塞和硬化截断），以评估肠道淋巴阻塞如何在 7、21 和 61 天时改变肠道微生物组。与对照组相比，ATLAS 后 7 天观察到肠道微生物组内的几个微生物亚群发生显着变化，包括普雷沃菌科和拟杆菌-普雷沃菌-卟啉单胞菌 (BPP) 丰度增加，这可能导致该肠道损伤模型中的疾病活动。据我们所知，这些发现代表了第一份将淋巴结构/肠道功能变化与肠道微生物组变化联系起来的报告，因为它们可能与 CD 的病理生理学有关。
The pathogenesis of inflammatory bowel disease (IBD) has been linked with lymphostasis, but whether and how lymphatic obstruction might disturb the intestinal microbiome in the setting of Crohn's Disease (CD) is currently unknown. We employed a new model of CD in African Green monkeys, termed 'ATLAS' (African green monkey truncation of lymphatics with obstruction and sclerosis), to evaluate how gut lymphatic obstruction alters the intestinal microbiome at 7, 21 and 61 days. Remarkable changes in several microbial sub- groupings within the gut microbiome were observed at 7 days post-ATLAS compared to controls including increased abundance of Prevotellaceae and Bacteroidetes-Prevotella-Porphyromonas (BPP), which may contribute to disease activity in this model of gut injury. To the best of our knowledge, these findings represent the first report linking lymphatic structural/gut functional changes with alterations in the gut microbiome as they may relate to the pathophysiology of CD.