EFFECTS OF PHAGOCYTOSIS AND COLCHICINE ON DISTRIBUTION OF LECTIN-BINDING SITES ON CELL-SURFACES
EFFECTS OF PHAGOCYTOSIS AND COLCHICINE ON DISTRIBUTION OF LECTIN-BINDING SITES ON CELL-SURFACES
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DOI：
10.1073/pnas.71.2.394
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
BERLIN, RD
中科院分区：
文献类型：
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作者：
OLIVER, JM;UKENA, TE;BERLIN, RD
研究了吞噬作用对凝集素与多形核白细胞质膜结合的影响。在分离的膜上测量了伴刀豆球蛋白A和蓖麻凝集素结合位点的比活性（定义为每100 μg膜蛋白结合的凝集素μg），它们与吞噬作用平行下降。我们的数据表明，这种去除发生的结合位点的浓度进入内化膜。秋水仙碱和长春碱，这并不抑制吞噬作用，防止从表面的凝集素结合位点的选择性去除。还表明，在37°下，凝集素诱导其自身的内化。这一性质被用来定义操作三类凝集素受体，其中之一是最广泛的吞噬过程中从质膜上删除。基于其他形态学的研究，它表明，在吞噬前的刀豆球蛋白结合位点的表面分布是随机的，它被推断，吞噬作用改变表面形貌通过诱导选择性运动的结合位点进入膜进行内化和秋水仙素敏感蛋白质是必不可少的这种强加的地形重组。
The effect of phagocytosis on lectin binding to plasma membranes of polymorphonuclear leukocytes was examined. The specific activities of binding sites of concanavalin A andRicinus communisagglutinin (defined as μg of lectin bound per 100 μg of membrane protein) were measured on isolated membranes; they decreased in parallel with phagocytosis. Our data suggest that this removal occurs by concentration of binding sites into internalized membrane. Colchicine and vinblastine, which did not inhibit phagocytosis, prevented the selective removal of lectin-binding sites from the surface. It was also shown that at 37° lectins induce their own internalization. This property was used to define operationally three classes of lectin receptors, one of which is most extensively removed from plasma membrane during phagocytosis. Based on other morphological studies in which it is shown that before phagocytosis the surface distribution of concanavalin-binding sites is random, it is inferred that phagocytosis alters surface topography by inducing the selective movement of binding sites into membrane undergoing internalization and that colchicine-sensitive proteins are essential for this imposed topographical reorganization.