Microscopical descriptions and chemical analysis by HPTLC of Taraxacum officinale in comparison to Hypochaeris radicata: a solution for mis-identification
Microscopical descriptions and chemical analysis by HPTLC of Taraxacum officinale in comparison to Hypochaeris radicata: a solution for mis-identification
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DOI：
10.1016/j.bjp.2014.07.018
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发表时间：
2014-08-01
期刊：
影响因子：
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通讯作者：
Osorio, Edison
中科院分区：
文献类型：
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作者：
Cortés, Natalie;Mora, Cristina;Osorio, Edison
蒲公英H. Wigg，菊科，经常被误认为或被Hypochaeris radicata L.，菊科（假菊科）。为了加深对T. officinale，并与H. radicata，我们调查了这两个物种的分类学，显微镜和色谱研究相结合，通过指纹图谱。微形态特征进行了研究，采用扫描电子显微镜，而光学显微镜用于组织化学观察。使用HPTLC构建指纹图谱。T. officinale被发现具有形态上不同类型的多细胞毛状体，其可用于区分两个物种，因为这些结构在H.胚根样本。此外，两种类型的乳管也可能是T.物种层次的植物。此外，HPTLC数据来自H. radicata和T. officinale根显示出明显不同的化学特征。本研究证实了T. officinale，所观察到的参数可以帮助最大限度地减少草药中的药物替代。(C)2014年，巴西药妆协会。出版社：Elsevier Editora Ltda All rights reserved.
Taraxacum officinale F. H. Wigg, Asteraceae, is frequently misidentified or substituted with Hypochaeris radicata L., Asteraceae (false dandelion). To increase our knowledge of T. officinale and differentiate it from H. radicata, we investigated the two species using a combination of taxonomy, microscopy, and chromatographic studies via fingerprint profiles. Micromorphological characteristics were studied using scanning electron microscopy, while optic light microscopy was used for histochemical observations. Fingerprint profiles were constructed using HPTLC. T. officinale was found to have a morphologically distinct type of pluricellular trichomes that can be used to differentiate the two species, as these structures were not identified in H. radicata samples. Furthermore, two types of laticiferous vessels may also be distinctive characteristics of T. officinale at species level. In addition, the HPTLC data derived from methanolic extracts of H. radicata and T. officinale roots showed clearly different chemical profiles. Thus this study establishes the authenticity of T. officinale, and the observed parameters could help minimize drug substitutions in herbal medicines. (C) 2014 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved.