Effect of salt intake on jejunal dopamine, Na+,K+-ATPase activity and electrolyte transport.
Effect of salt intake on jejunal dopamine, Na+,K+-ATPase activity and electrolyte transport.
复制标题
盐摄入量对空肠多巴胺、Na,K-ATP酶活性和电解质转运的影响。
DOI：
10.1046/j.1365-201x.2000.00656.x
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Patrício Soares
中科院分区：
文献类型：
--
作者：
V. Lucas;M. Serrão;Patrício Soares
本研究探讨了盐摄入与空肠多巴胺、Na+、K+- atp酶活性和电解质运输的相关性。低盐，而不是高盐，摄入2周后空肠黏膜多巴胺水平增加，同时l -3,4-二羟基苯丙氨酸组织水平显著降低。相比之下，在禁食72 h的大鼠中，低盐饮食再喂养24 h的效果没有改变多巴胺组织水平，但显著增加了l -3,4-二羟基苯丙氨酸。相比之下，高盐摄入显著增加了多巴胺和l -3,4-二羟基苯丙氨酸的组织水平，而多巴胺/ l -3,4-二羟基苯丙氨酸的组织比例没有变化。对照组（正常盐摄取量2周）大鼠组织中l -3,4-二羟基苯丙氨酸和多巴胺水平均显著高于禁食72 h再喂养24 h的大鼠（P < 0.05）。禁食72 h再饲喂24 h后，空肠Na+、K+- atp酶活性明显降低，在再饲喂期间饲喂正常盐和高盐日粮的大鼠尤为明显。饲喂正常盐饮食2周24 h的大鼠基础短路电流相似，饲喂正常盐饮食、低盐饮食和高盐饮食后，基础短路电流没有改变。另一方面，长期低盐摄入对空肠Na+、K+- atp酶活性和基础短路电流的影响显著降低，而高盐摄入对空肠Na+、K+- atp酶活性和基础短路电流没有影响。在高盐饮食喂养2周的大鼠中发现，与正常和低盐饮食喂养的大鼠相比，瓦巴因在减少空肠短路电流方面更有效。与正常盐摄入量的大鼠相比，在高盐和低盐饮食喂养2周的大鼠中，呋塞米的效果更为明显。在正常、低盐和高盐饮食喂养2周和24小时的大鼠中，多巴胺（高达1微mol L-1）对空肠上皮细胞Na+、K+- atp酶和基础短路电流没有改变。
The present study addresses the question of the relevance of salt intake on jejunal dopamine, Na+,K+-ATPase activity and electrolyte transport. Low salt, but not high salt, intake for 2 weeks increased dopamine levels in the jejunal mucosa accompanied by a marked decrease in L-3,4-dihydroxyphenylalanine tissue levels. By contrast, in rats fasted for 72 h the effect of refeeding for 24 h with a low salt diet failed to change dopamine tissue levels, although it significantly increased those of L-3,4-dihydroxyphenylalanine. By contrast, high salt intake markedly increased the tissue levels of both dopamine and L-3,4-dihydroxyphenylalanine, without changes in dopamine/L-3,4-dihydroxyphenylalanine tissue ratios. Tissue levels of both L-3,4-dihydroxyphenylalanine and dopamine in control conditions (normal salt intake for 2 weeks) were markedly higher (P < 0.05) than in rats submitted to 72 h fasting plus 24 h refeeding. The effect of fasting for 72 h followed by 24 h refeeding was a marked decrease in jejunal Na+,K+-ATPase activity, particularly evident for rats fed a normal salt and high salt diets during the refeeding period. Basal short circuit current was similar in rats fed a normal salt diet for 2 weeks and 24 h, and the type of diet failed to alter basal short circuit current after refeeding with normal, low and high salt diets. On the other hand, the effect of prolonged low salt intake was a marked decrease in jejunal Na+, K+-ATPase activity and basal short circuit current, whereas high salt intake failed to alter enzyme activity and basal short circuit current. In rats fed for 2 weeks a high salt diet ouabain was found to be more potent in reducing jejunal short circuit current than in rats fed normal and low salt diets. The effect of furosemide was more marked in rats fed for 2 weeks high and low salt diets than in animals receiving a normal salt intake. Dopamine (up to 1 micromol L-1) was found not to alter Na+,K+-ATPase and basal short circuit current in jejunal epithelial sheets, in rats fed with normal, low and high salt diets for 2 weeks and 24 h.
DOI：
10.1152/ajprenal.1990.258.1.f52
发表时间：
1990
期刊：
The American journal of physiology
影响因子：
--
作者：
I. Seri;B. Kone;S. Gullans;A. Aperia;B. Brenner;B. Ballermann
通讯作者：
I. Seri;B. Kone;S. Gullans;A. Aperia;B. Brenner;B. Ballermann