Pulsed electron-nuclear double resonance (ENDOR) at 140 GHz
Pulsed electron-nuclear double resonance (ENDOR) at 140 GHz
复制标题
DOI：
10.1006/jmre.1999.1727
复制
发表时间：
1999-06-01
影响因子：
2.2
通讯作者：
Griffin, RG
中科院分区：
文献类型：
--
作者：
Bennati, M;Farrar, CT;Griffin, RG
我们描述了一个光谱仪的脉冲ENDOR在140 GHz，这是基于微波雪崩二极管放大器和探头组成的TE 011腔与高品质的谐振电路可变射频照射。对于脉冲EPR，我们在20 K下获得3 × 10（9）自旋/高斯的绝对灵敏度。用标准的掺杂到聚苯乙烯中的双苯撑苯基烯丙基（BDPA）和来自E.大肠杆菌核糖核苷酸还原酶（RNR）。RNR酪氨酰基自由基的EPR谱在5 T下显示出显著的g-各向异性，并用于证明取向选择性Davies-ENDOR。(C)北京：科学出版社.
We describe a spectrometer for pulsed ENDOR at 140 GHz, which is based on microwave IMPATT diode amplifiers and a probe consisting of a TE011 cavity with a high-quality resonance circuit for variable radiofrequency irradiation. For pulsed EPR we obtain an absolute sensitivity of 3 x 10(9) spins/Gauss at 20 K. The performance of the spectrometer is demonstrated with pulsed ENDOR spectra of a standard bis-diphenylene-phenyl-allyl (BDPA) doped into polystyrene and of the tyrosyl radical from E. coli ribonucleotide reductase (RNR). The EPR spectrum of the RNR tyrosyl radical displays substantial g-anisotropy at 5 T and is used to demonstrate orientation-selective Davies-ENDOR. (C) 1999 Academic Press.