Magnetic Mixed Micelles Composed of a Non-Ionic Surfactant and Nitroxide Radicals Containing a d-Glucosamine Unit: Preparation, Stability, and Biomedical Application
Magnetic Mixed Micelles Composed of a Non-Ionic Surfactant and Nitroxide Radicals Containing a d-Glucosamine Unit: Preparation, Stability, and Biomedical Application
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DOI：
10.3390/pharmaceutics11010042
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发表时间：
2019-01
期刊：
影响因子：
5.4
通讯作者：
Kota Nagura;Yusa Takemoto;Fumi Yoshino;A. Bogdanov;N. Chumakova;A. Vorobiev;H. Imai;Tetsuya Matsuda;Satoshi Shimono;Tatsuhisa Kato;N. Komatsu;R. Tamura
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文献类型：
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作者：
Kota Nagura;Yusa Takemoto;Fumi Yoshino;A. Bogdanov;N. Chumakova;A. Vorobiev;H. Imai;Tetsuya Matsuda;Satoshi Shimono;Tatsuhisa Kato;N. Komatsu;R. Tamura
在pH 7.4的磷酸盐缓冲溶液(PBS)中，将生物相容的非离子表面活性剂吐温80与含有D-氨基葡萄糖的无毒疏水的吡咯烷-N-氧基自由基混合，制备了无金属磁性混合胶束(平均直径约20 nm)。混合胶束的时间-过程稳定性和体外磁共振成像(MRI)对比能力被发现依赖于氮氧自由基烷基链的长度。还证实了混合胶束在体内和体外都没有毒性，并且在大量过量抗坏血酸存在下具有很高的稳定性。体内核磁共振实验表明，其中一个混合胶束在质子纵向弛豫时间(T1)加权图像中表现出比我们先前报道的其他磁性混合胶束更高的对比度增强。因此，本文提出的磁性混合胶束有望成为治疗嗅觉纳米药物的造影剂，例如磁共振可见的靶向药物输送载体。
Metal-free magnetic mixed micelles (mean diameter: < 20 nm) were prepared by mixing the biocompatible non-ionic surfactant Tween 80 and the non-toxic, hydrophobic pyrrolidine-N-oxyl radicals bearing a d-glucosamine unit in pH 7.4 phosphate-buffered saline (PBS). The time-course stability and in vitro magnetic resonance imaging (MRI) contrast ability of the mixed micelles was found to depend on the length of the alkyl chain in the nitroxide radicals. It was also confirmed that the mixed micelles exhibited no toxicity in vivo and in vitro and high stability in the presence of a large excess of ascorbic acid. The in vivo MRI experiment revealed that one of these mixed micelles showed much higher contrast enhancement in the proton longitudinal relaxation time (T1) weighted images than other magnetic mixed micelles that we have reported previously. Thus, the magnetic mixed micelles presented here are expected to serve as a promising contrast agent for theranostic nanomedicines, such as MRI-visible targeted drug delivery carriers.