A MONOCLONAL-ANTIBODY TO VASOPRESSIN - PREPARATION, CHARACTERIZATION, AND APPLICATION IN IMMUNOCYTOCHEMISTRY
A MONOCLONAL-ANTIBODY TO VASOPRESSIN - PREPARATION, CHARACTERIZATION, AND APPLICATION IN IMMUNOCYTOCHEMISTRY
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DOI：
10.1177/30.12.6185560
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发表时间：
1982-01-01
影响因子：
3.2
通讯作者：
ZIMMERMAN, EA
中科院分区：
文献类型：
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作者：
HOUYU, A;EHRLICH, PH;ZIMMERMAN, EA
下丘脑-神经垂体系统包含催产素 (OT) 和血管加压素 (VP) 及其相关载体蛋白神经生理素 (NPS)，是 40 多年来广泛研究的主题。在过去十年中，通过应用免疫细胞化学方法（使用针对激素和 NPS 的兔抗血清）对该系统进行了重新研究。 VP单克隆抗体的制备、表征及其在免疫组化中的应用。该抗体不与 OT 或精氨酸加压素 (AVT) 发生交叉反应。其抗原决定簇以各种 VP 类似物吸收为特征，包括 2 个芳香族氨基酸：3 位的 Phe，2 位的 Tyr。与戊二醛相比，用甲醛固定组织导致免疫染色不充分，很可能是由于前一种固定剂干扰芳香族氨基酸决定簇。
The hypothalamo-neurohypophysial system, containing the hormones oxytocin (OT) and vasopression (VP) and their associated carrier proteins, the neurophysins (NPS), was the subject of extensive investigation for more than 40 yr. This system was reinvestigated during the last decade by application of immunocytochemical methods employng the rabbit antisera to the hormones and NPS. The preparation and characterization of a monoclonal antibody to VP and its application in immunohistochemistry. The antibody did not cross-react with OT or arginine vasotocin (AVT). Its antigenic determinants as characterized by absorption with various VP analogs included 2 aromatic amino acids: Phe in position 3, and to a lesser extent Tyr in 2. Tissue fixation with formaldehyde resulted in inadequate immunostaining as compared to glutaraldehyde, most likely due to interference with the aromatic amino acid determinants by the former fixative.